| 枯草芽胞杆菌异戊二烯酶ComQ的生物信息学分析及对生物膜形态的影响 |
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| 引用本文： | 李伟,吴静,冯静静,汪剑,周梦洁,胡汶松,Sokhna mbacke Gningue,薛正莲,赵世光,钱森和,王洲,刘艳.枯草芽胞杆菌异戊二烯酶ComQ的生物信息学分析及对生物膜形态的影响[J].微生物学报,2022,62(2):533-542. |
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| 作者姓名： | 李伟 吴静 冯静静 汪剑 周梦洁 胡汶松 Sokhna mbacke Gningue 薛正莲 赵世光 钱森和 王洲 刘艳 |
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| 作者单位： | 安徽工程大学生物与食品工程学院, 安徽 芜湖 241000;安徽工程大学生物与食品工程学院, 安徽 芜湖 241000;安徽省工业微生物分子育种工程实验室, 安徽 芜湖 241000 |
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| 基金项目： | 国家自然科学基金（31871781，31772081）；安徽高校自然科学研究重点项目（KJ2018A0106）；芜湖市科技计划重点项目（2020yf62）；国家级大学生创新创业项目（201910363042，201810363046）；安徽省大学生创新创业计划项目（201710363178） |
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| 摘 要： | 目的]枯草芽胞杆菌ComQ是一种类异戊二烯生物合成酶.利用生物信息学预测分析了ComQ的生物学特性,对comQ基因进行过表达和敲除,构建突变菌,孔板发酵培养验证生物膜形态变化.方法]运用NCBI (National Center for Biotechnology Information)网站里的Protein数据...
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| 关 键 词： | 枯草芽胞杆菌 生物信息学分析 生物膜 过表达 敲除 |
| 收稿时间： | 2021/3/27 0:00:00 |
| 修稿时间： | 2021/5/20 0:00:00 |
Bioinformatics analysis of Bacillus subtilis isoprene enzyme ComQ and its effect on biofilm morphology |
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| LI Wei,WU Jing,FENG Jingjing,WANG Jian,ZHOU Mengjie,HU Wensong,Sokhna mbacke Gningue,XUE Zhenglian,ZHAO Shiguang,QIAN Senhe,WANG Zhou,LIU Yan.Bioinformatics analysis of Bacillus subtilis isoprene enzyme ComQ and its effect on biofilm morphology[J].Acta Microbiologica Sinica,2022,62(2):533-542. |
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| Authors: | LI Wei WU Jing FENG Jingjing WANG Jian ZHOU Mengjie HU Wensong Sokhna mbacke Gningue XUE Zhenglian ZHAO Shiguang QIAN Senhe WANG Zhou LIU Yan |
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| Institution: | College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China;College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China;Anhui Provincial Engineering Laboratory for Molecular Breeding of Industrial Microbial, Wuhu 241000, Anhui, China |
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| Abstract: | Objective] Bacillus subtilis ComQ is a kind of isoprene biosynthesis enzyme. We used bioinformatics to predict and analyze the biological characteristics of ComQ. In addition, we also constructed comQ overexpression and knockout mutants to verify their effect on biofilm morphology. Methods] Firstly, we obtained the amino acid sequence of ComQ protein from the Protein database in the NCBI website. Secondly, we predicted and analyzed the biological characteristics including physical and chemical properties, signal peptide, domain and spatial structure of ComQ by online bioinformatics software. Thirdly, we constructed comQ overexpression and knockout strain of Bacillus subtilis, and observe their growth characteristics of biofilm by plate fermentation. Results] ComQ protein was composed of 299 amino acids with a theoretical molecular weight of about 34 204.08 Da. It was a stable intracellular protein without signal peptide and transmembrane region. Colony PCR verified that Bacillus subtilis BS168 mutants were constructed successfully. The biofilm morphology of the two mutants was different from that of the original strain BS168. Conclusion] Through the prediction and analysis of the basic properties, key amino acid sites and protein structure of ComQ protein, the mutant strain was constructed to verify the morphological changes of biofilm, which laid a foundation for the subsequent study of ComQ on the growth and metabolism of Bacillus subtilis. |
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| Keywords: | Bacillus subtilis bioinformatics analysis biofilm overexpression knockout |
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