透明颤菌血红蛋白基因表达对金色链霉菌生长代谢的影响

利用四环素抗性基因启动子在金色链霉菌中表达透明颤菌血红蛋白基因。在1m3发酵罐中研究了工程菌株的生长代谢特性。在溶解氧充足的条件下，透明颤菌血红蛋白表达，对金色链霉菌生长代谢未产生明显影响，工程菌株与参比菌株的生长代谢特性基本一致，工程菌株和参比菌株金霉素最终浓度分别为22905u/mL、22896u/mL。在低溶解氧条件下，透明颤菌血红蛋白的表达，可促进金色链霉菌菌体生长、菌丝活力保持和金霉素的合成：工程菌菌体浓度比参比菌株高5％～10％，产物合成提高11.4％。

Study on Effeet of Vitreoscilla Hemoglobin Gene Expression on Growth and Metabolism of Streptomyces aureofaciens

Vitreoscilla hemoglobin gene was expressed in S. aureofaciens through promoter of tetracycline resistance gene. Characteristics of S. aureofaciens growth and metabolism were studied in 1 m3 fermenter. In high dissolved oxygen concentrations, expression of hemoglobin gene had little effect on growth and metabolism of S. aureofaciens, and there were no obvious differences between engineering strain and control. The chlortetracycline of engineering strain was 22905 u/mL and of control was 22896 u/mL respectively. Under conditions of low dissolved oxygen, expression of hemoglobin enhanced growth, maintenance of energetic mycelium configuration and chlortetracycline yield of S. aureofaciens: the mycelium concentrations of engineering strain were more about 5%-10% and yield was more 11.4% than control.