| 古尼虫草的生物活性物质I含肽镇痛组分的分离及性质 |
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| 引用本文： | 朱振元,梁宗琦,常胜军,刘爱英.古尼虫草的生物活性物质I含肽镇痛组分的分离及性质[J].微生物学报,2002,42(6):732-736. |
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| 作者姓名： | 朱振元 梁宗琦 常胜军 刘爱英 |
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| 作者单位： | 1. 贵州大学生物技术学院,真菌资源研究室,贵阳,550025;浙江大学农业与生物技术学院,杭州,310029
2. 贵州大学生物技术学院,真菌资源研究室,贵阳,550025 |
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| 基金项目： | 贵州省“九五”攻关课题 ( 95D96A -1 39) |
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| 摘 要： | 用理化分离分析和生物检测方法相结合，从古尼虫草(Cordyceps gunnii(Berk.)Berk.)无性型，古尼拟青霉(Paecilomyces gunnii Liang)菌丝体中初步分离纯化得到镇痛物质，该物质经氨基酸组成分析表明是一种酸性氨基酸残基高的肽类物质。经不同温度、pH及蛋白酶的稳定性试验分析观察到这种肽类物质对酸稳定、在酸性条件下抗热，对胃蛋白酶、胰蛋白酶部分敏感，对蛋白酶K不敏感。经小鼠竖尾法和大鼠攻击法测定，无吗啡类药物依赖性。
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| 关 键 词： | 古尼虫草，古尼拟青霉，镇痛活性成分，药物依赖性 |
| 文章编号： | 0001-6209(2002)06-0732-05 |
Bioactive Substances from Cordyceps gunnii I. Separation,Purification and Properties of Analgesic Substance |
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| Zhu Zhenyuan , Liang Zongqi Chang Shengjun Liu Aiying.Bioactive Substances from Cordyceps gunnii I. Separation,Purification and Properties of Analgesic Substance[J].Acta Microbiologica Sinica,2002,42(6):732-736. |
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| Authors: | Zhu Zhenyuan Liang Zongqi Chang Shengjun Liu Aiying |
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| Institution: | Zhu Zhenyuan 1,2 Liang Zongqi 1 Chang Shengjun 1 Liu Aiying 1 |
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| Abstract: | The analgesic substance from mycelia of Paecilomyces gunnii Liang that is anamorph of Cordyceps gunnii(Berk.)Berk. was isolated and purified by combined with separation and analyzing and biology experiment. Based on the results by amino acid analyzer showed that the analgesic substance from P.gunnii is not dissociate amino acid but a bioactive peptide consisting of more acidic amino acids .The analgesic bioactive substance was stable to acids and host under acidic condition. It was not sensitive to proteinase K and partly sensitive to pepsin and trypsin. By animal tests of Mus musculus and Rattus norxegius showed the analgesic bioactive substance have no drug addiction being similar to morphia. |
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| Keywords: | Cordyceps gunnii Paecilomyces gunnii Analgesic bioactive substance Drug addiction |
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