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太平洋帕里西维拉海盆细菌多样性的非培养的初步分析
引用本文:谢华,薛燕芬,赵爱民,李铁钢,马延和.太平洋帕里西维拉海盆细菌多样性的非培养的初步分析[J].微生物学报,2005,45(1):1-5.
作者姓名:谢华  薛燕芬  赵爱民  李铁钢  马延和
作者单位:1. 中国科学院微生物研究所,北京,100080;中国科学院研究生院,北京,100039
2. 中国科学院微生物研究所,北京,100080
3. 中国生物技术发展中心,北京,100081
4. 中国科学院海洋研究所,青岛,266071
基金项目:国家“863计划”( 2 0 0 1AA62 60 40 )~~
摘    要:从环境中直接提取总DNA ,构建了含 32个有效转化子的太平洋帕里西维拉 (PareceVela)海盆 5 0 10米深处底泥的细菌 16SrRNA基因文库。测序结果表明 ,可以将 32条有效序列分为 17个不同的分类单元。大部分序列与已知细菌类群的 16SrDNA序列相似性较高 ,归属于Proteobacteria的gamma亚群、alpha亚群和海洋非培养细菌类群 ,主要分布在Pseudoalteromonas属、Halomonas属、Alcanivorax属、Photobacterium属、Acinetobacter属 ;部分序列与已知细菌类群的 16SrDNA序列同源性较低 ,可能代表新的分类单位。研究结果表明 ,帕里西维拉海盆不仅含有丰富的微生物物种 ,并且存在尚未被认识的新物种

关 键 词:深海  生物多样性  非培养方法
文章编号:0001-6209(2005)01-0001-05
修稿时间:2004年4月29日

Preliminary research on bacterial diversity of Parece vela Basin,Pacific Ocean by culture-independent method
XIE Hua , XUE Yan-fen Zhao Ai-min LI Tie-gang MA Yan-he.Preliminary research on bacterial diversity of Parece vela Basin,Pacific Ocean by culture-independent method[J].Acta Microbiologica Sinica,2005,45(1):1-5.
Authors:XIE Hua  XUE Yan-fen Zhao Ai-min LI Tie-gang MA Yan-he
Institution:Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. huaxie399@hotmail.com
Abstract:The environmental DNA was directly extracted from the sediment in Parece Vela Basin, Pacific Ocean, at a depth of 5010m. Bacterial 16S rRNA gene library of 32 clones was generated using bacterial universal primers and 16S rDNA sequences were analyzed phylogenetically. 17 phylotypes were obtained. The library was dominated by gamma-Proteobacteria, alpha-Proteobacteria and marine uncultured bacteria. Sixty-two percent of the cloned sequences was highly related to the known bacteria in the genus Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, Pseudoalteromonas (>96% sequence similarity),while some of the cloned sequences showed less affiliation with known taxa (<94% sequence similarity) and may represent novel taxa.
Keywords:Deep-sea  Culture-independent  Bacterial biodiversity
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