酵母菌中表达的新疆家蚕抗菌肽（Cecropin-XJ）的特性研究

研究酵母菌中表达的新疆家蚕抗菌肽 (CecropinXJ)的抗菌特性。根据琼脂孔穴扩散法，检测抗菌肽的热稳定性、抑菌效价、对氨苄青霉素抗性菌的抑菌作用，并检测了抗菌肽对酸碱盐、人造胃液的耐受性及其抗菌谱。结果显示新疆家蚕抗菌肽具有很强的热稳定性、能够杀灭氨苄青霉素抗性菌、对酸碱盐、人造胃酸有一定的耐受性，其杀菌活力为：1mg抗菌肽与1200U的氨苄青霉素杀菌活力相当。新疆家蚕抗菌肽能够不同程度地杀灭革兰氏阳性菌和革兰氏阴性菌，这一发现将为抗菌肽在农业、医疗卫生、畜牧业等方面的应用奠定基础，同时为深入研究抗菌肽作用机制提供了依据。

Studies on the Properties of Cecropin-XJ Expressed in Yeast from Xinjiang Silkworm

The purpose of this study is to investigate the properties of recombinant Cecrop in-XJ isolated from Xinjing silkworm and expressed in Pichia yeast. Accordi ng to Agarose Diffusion Assay, this recombinant Cecropin-XJ has exhibited an ex treme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus and S. enterica. Moreover, we have observed that the Cecropin -XJ well tolerant to extreme acidic, basic, and high salt environments as well as resistant to 24 hours digestion by artificial gastric juice. The inhibition c apability to S. aureus with 1mg Cecropin-XJ is equal to 1200U ampicillin. W ith a broad spectrum of antibacterial activities, the Cecropin-XJ is able to in hibit the Gram-positive bacteria and Gram-negative bacteria. These findings co uld lead it to a broad applications for agriculture, medical, domestic animal an d food industry. The further investigation of the antibacterial mechanism of cec ropin-XJ is needed.