基于高通量测序的不同虫态洋虫内共生微生物群落分析

【目的】为分析不同虫态下洋虫内共生微生物菌群组成情况,以成虫和幼虫两种虫态为研究对象。【方法】通过对洋虫内共生微生物提取及高通量测序分析,获得不同样品中微生物组成结构及变化情况。【结果】不同虫态下,随着生长发育的进行,洋虫体内细菌在丰度和多样性两个方面都有很大变化,成虫中的菌群数量相对较少,多样性较幼虫也明显降低。在几种特定微生物组成上不同虫态也存在着较大的差异,成虫中,漫游球菌属和乳酸菌菌属为优势菌属;幼虫体内乳酸菌菌属为优势菌属。真菌变化不明显,孢囊线黑粉酵母菌属为其优势菌属。【结论】研究结果将为后继洋虫生物资源的研究提供相关信息,为洋虫内共生微生物资源开发与利用提供理论依据。

Microbial diversity in Martianus dermestoides at different stages based on high-throughput sequencing technology

Objective] To analyze the composition of endosymbiosis microbes of Martianus dermestoides at different stages, we took adult and larva as the research objects. Methods] By the extraction of endosymbiosis microbes of Martianus dermestoides and high-throughput sequencing analysis, we obtained the composition and changes of microflora in different samples. Results] At different insect stages, the endosymbiosis microbes abundance and diversity of Martianus dermestoides changed greatly with the development of the insect, the number of bacteria in the adult was relatively small, and the diversity was significantly lower than that in the larva. Different insect states had also significant differences in the composition of several specific microorganisms. Among the adult, Vagococcus and Lactobacillus were dominant bacterial genus; Lactobacillus in larvae was the dominant bacterial genus. The change of fungi was not obvious, and Saccharomyces spp. was dominant. Conclusion] Our findings provide related information of biotic resources of Martianus dermestoides. Meanwhile, the result can also furnish the theoretical basis for the development and utilization of Martianus dermestoides endosymbiosis microorganism resources.