西藏扎布耶茶卡盐碱湖古菌多样性的非培养技术分析

采用非培养技术，直接从西藏扎布耶茶卡盐碱湖样品中提取微生物总DNA。以样品总DNA为模板，PCR扩增湖中古菌的16S rDNA序列。扩增产物经过克隆并随机挑选60个克隆进行测序得到它们的16S rDNA部分序列，大部分序列与嗜盐碱古菌的16S rDNA相近。在系统发育树上，部分克隆与已知古菌属归于同一分支，主要分布在嗜盐菌科的Natronococcus、Natronorubrum、Natronobacterium、Natronomonas、Natrinema、Halorubrum、Haloterrigena和Halorhabdus等8个嗜盐古菌属中, 也有一些克隆形成了独立的分支。它们共同显示出扎布耶茶卡湖中的古菌具有丰富的多样性。

Archaeal Diversity of Zabuye Lake in Tibet Analyzed by Culture-independent Approach

With culture-independent approach, microbial DNA was directly extracted from samples of Zabuye saline soda lake. Using the microbial DNA as template, archaeal 16S rDNAs were amplified by PCR. Amplified products were cloned and sequenced. 60 different cloned partial sequences, most of which were related to haloalkaliphilic archaeon, were acquired. In the phylogenetic tree, some clones of Zabuye lake belonged to Genus Natronobacterium,Natrinema,Natronococcus,Natronorubrum,Natronomonas,Halorubrum,Haloterrigena,Halorhabdus in Family Halobacteriaceae. Other clones represent some novel groups. All of them show prolific archaeal diversity of Zabuye lake.