| 枯草芽孢杆菌α乙酰乳酸脱羧酶基因在啤酒酵母工业菌株中的表达 |
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| 引用本文： | 郭文洁,何秀萍,铁翠娟,张博润.枯草芽孢杆菌α乙酰乳酸脱羧酶基因在啤酒酵母工业菌株中的表达[J].微生物学报,2001,41(1):105-108. |
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| 作者姓名： | 郭文洁 何秀萍 铁翠娟 张博润 |
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| 作者单位： | 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 国家自然科学基金资助项目(39970010) |
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| 摘 要： | 啤酒酿造中,双乙酰是影响啤酒生产熟化期长短及其风味的主要因素.存在于多种细菌中的a-乙酰乳酸脱羧酶(EC4.1.1.5,简称a-ALDC)1]能将双乙酰的前体a-乙酰乳酸直接转化为对啤酒风味没有影响的乙偶姻,从而大大降低啤酒中双乙酰的含量,缩短啤酒熟化期.但所有的啤酒酵母菌不产生此酶.虽然在发酵过程中添加此酶是一个解决的途径,但解决问题的根本是将ALDC基因引入到啤酒酵母菌中.国外已开展这方面的研究2,3],本研究组曾用随机克隆的方法获得了枯草芽孢杆菌a-ALDC基因4],本文报道了枯草芽孢杆菌ALDC基因在工业用啤酒酵母中的表达研究结果.
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| 关 键 词： | α－乙酰乳酸脱羧酶 基因表达 啤酒酵母 工业菌株 表达 |
| 文章编号： | 0001-6209(2001)01-0105-04 |
Expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis in Brewer's yeast] |
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| W Guo,X He,C Tie,B Zhang.Expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis in Brewer''s yeast][J].Acta Microbiologica Sinica,2001,41(1):105-108. |
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| Authors: | W Guo X He C Tie B Zhang |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
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| Abstract: | Yeast YIp-type expression recombinant plasmid(pCMA2-1) was constructed. The expression of alpha-acetolactate decarboxylase gene from Bacillus subtilis was controled by CUP1 promoter and its own terminator. The recombinant plasmid pCMA2-1 was introduced into the brewer's yeast PJ3-5. Transformants were selected using copper resistance as selected marker. The results of activity assay showed that PJ3-5 didn't produce alpha-acetolactate decarboxylase(ALDC), where as the activity of alpha-ALDC in transformants were induced by copper sulfate. The laboratory scale fermentation test confirmed that the total diacetyl concentration was reduced effectively by alpha-ALDC in transformant. |
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| Keywords: | acetolactate decarboxylase Gene expression Brewer's yea$ |
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