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| 一株抗水稻纹枯病菌的解淀粉芽胞杆菌分离与鉴定 | |
| 引用本文： | 朱晓飞,张晓霞,牛永春,胡元森,闫艳春,王海胜.一株抗水稻纹枯病菌的解淀粉芽胞杆菌分离与鉴定[J].微生物学报,2011,51(8):1128-1133. |
| 作者姓名： | 朱晓飞 张晓霞 牛永春 胡元森 闫艳春 王海胜 |
| 作者单位： | 1. 河南工业大学生物工程学院,郑州,450001 2. 中国农业科学院农业资源与农业区划研究所,北京,100081 3. 中国农业科学院研究生院,北京,100081 |
| 基金项目： | 中国农业科学院基本科研业务基金项目(0042009001) |
| 摘 要： | 【目的】筛选对水稻纹枯病菌(Rhizoctonia solani)具有强拮抗作用的细菌菌株。【方法】用指示菌法筛选拮抗菌株;通过形态观察、生理生化实验、Biolog及16S rDNA序列分析鉴定目标菌株;利用平板双向培养法和滤纸片扩散法测定抑菌谱及拮抗性质。【结果】分离到一株高活力的水稻纹枯病菌拮抗菌株YB-3,该菌株属于解淀粉芽胞杆菌(Bacillus amyloliquefaciens);菌株YB-3对常见的14株病原真菌和7株细菌具有较强的拮抗作用,并发现其对亲缘关系较近的芽孢菌属有较强的拮抗作用;该菌株的抑制活性具有温度稳定、耐酸、但对蛋白酶敏感的特点。【结论】通过指示菌法筛选到一株对水稻纹枯病菌有强拮抗作用的解淀粉芽胞杆菌(B.amyloliquefaciens)YB-3,它具有广谱、高效的植物病原菌拮抗活性。 |
| 关 键 词： | 解淀粉芽胞杆菌 鉴定 拮抗特性 生物防治 |
| 收稿时间： | 2011/1/14 0:00:00 |
| 修稿时间： | 2011/4/19 0:00:00 |
Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani |
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| Xiaofei Zhu,Xiaoxia Zhang,Yongchun Niu,Yuansen Hu,Yanchun Yan and Haisheng Wang.Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani[J].Acta Microbiologica Sinica,2011,51(8):1128-1133. | |
| Authors: | Xiaofei Zhu Xiaoxia Zhang Yongchun Niu Yuansen Hu Yanchun Yan and Haisheng Wang |
| Institution: | Xiaofei Zhu1,Xiaoxia Zhang3,Yongchun Niu3,Yuansen Hu1,Yanchun Yan2,Haisheng Wang2* 1Institute of Bioengineering of Henan University of Technology,Zhengzhou 450001,China 2Chinese Academy of Agricultural Sciences,Beijing 100081,China 3Institute of Agricultural Resources and Regional Planning CAAS,China |
| Abstract: | Objective] An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils.Methods] Antagonistic strains were isolated by a reporter strain method.YB-3 was identified based on morphology observation,physiological and biochemical characterizations,Biolog,G+C content and 16S rDNA sequence analysis.The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate tw... |
| Keywords: | Bacillus amyloliquefaciens identification antagonistic properties biological control |
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