| 溃烂症眼斑拟石首鱼分离的灿烂弧菌的鉴定 |
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| 引用本文： | 马爱敏,闫茂仓,胡利华,谢起浪,陈少波,单乐州,邵鑫斌.溃烂症眼斑拟石首鱼分离的灿烂弧菌的鉴定[J].微生物学报,2010,50(4):542-547. |
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| 作者姓名： | 马爱敏 闫茂仓 胡利华 谢起浪 陈少波 单乐州 邵鑫斌 |
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| 作者单位： | 浙江省海洋水产养殖研究所,温州325005;浙江省近岸水域生物资源开发与保护重点实验室,温州325005 |
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| 基金项目： | 浙江省科技厅科研院所专项公益技术攻关项目（2007F10009） |
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| 摘 要： | 摘要：【目的】从患溃烂症的眼斑拟石首鱼分离到优势菌株M-1，并进一步对该菌的系统发育学进行了分析。【方法】采用形态学、生理生化鉴定，结合16S rRNA和HSP60基因序列分析。【结果】16S rRNA基因同源性分析，菌株M-1与灿烂弧菌(AJ874361、AY046955)聚为一群；HSP60基因(groES)序列分析表明M-1与弧菌(EU653883、AY837440)聚为一个分支。【结论】菌株M-1属于灿烂弧菌(Vibrio splendidus)。
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| 关 键 词： | 关键词：M-1菌株 致病性 16S rRNA基因 HSP60基因 灿烂弧菌 |
| 收稿时间： | 2009/10/18 0:00:00 |
| 修稿时间： | 2010/1/18 0:00:00 |
Identification of a Vibrio splendidus strain isolated from diseased Sciaenops ocellatus |
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| Aimin M,Maocang Yan,Lihua Hu,Qilang Xie,Shaobo Chen,Lezhou Shan and Xinbin Shao.Identification of a Vibrio splendidus strain isolated from diseased Sciaenops ocellatus[J].Acta Microbiologica Sinica,2010,50(4):542-547. |
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| Authors: | Aimin M Maocang Yan Lihua Hu Qilang Xie Shaobo Chen Lezhou Shan and Xinbin Shao |
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| Institution: | Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China;Zhejiang Mariculture Research Institute, Wenzhou 325005, China |
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| Abstract: | Objective We isolated bacterial strain named M-1 from ill Sciaenops ocellatus with ulceration,and further analyzed the phylogenetics of the bacterial strain.MethodsWe characterized the strain on the basis of its morphology, physiology,and sequence comparison of 16S rRNA and HSP60 genes.ResultsStrain M-1 was identified as Vibrio splendidus.16SrRNA gene of strain M-1 and Vibrio splendidus(AJ874361 ) had nearest kinship,and the analysis of HSP60 gene sequence indicated that strain M-1 and Vibrio(EU653883,AY837... |
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| Keywords: | Keywords: Strain M-1 Pathogenicity 16S rRNA gene HSP60 gene Vibrio splendidus |
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