| 水稻条斑病菌gacA基因的克隆及功能初析 |
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| 引用本文： | 杨万风,陈磊,刘红霞,胡白石,刘凤权.水稻条斑病菌gacA基因的克隆及功能初析[J].微生物学报,2007,47(2):208-212. |
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| 作者姓名： | 杨万风 陈磊 刘红霞 胡白石 刘凤权 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室 南京 210095;南京农业大学农业部病虫监测与治理重点开放实验室 南京 210095;南京农业大学农业部病虫监测与治理重点开放实验室 南京 210095;南京农业大学农业部病虫监测与治理重点开放实验室 南京 210095;南京农业大学农业部病虫监测与治理重点开放实验室 南京 210095 |
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| 基金项目： | 国家自然科学基金(30070497);; 国家“863计划”(2002AA245041) |
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| 摘 要： | 根据黄单胞菌gacA基因的同源性设计简并引物,采用PCR方法从水稻条斑病菌(Xanthomonas oryzae pv.oryzicola,Xooc)中克隆了gacA同源基因,命名为gacAXooc。序列比较显示,该基因在黄单胞菌中是相对保守的。通过同源重组的方法,构建了gacAXooc的插入突变株。对0.1% Tryptone的趋化应答能力检测发现,gacA突变株的趋化能力明显降低,证明gacA与Xooc的趋化性相关。
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| 关 键 词： | 水稻条斑病菌 gacA基因 突变 趋化性 |
| 文章编号： | 0001-6209（2007）02-0208-05 |
| 收稿时间： | 7/4/2006 12:00:00 AM |
| 修稿时间： | 2006-07-04 |
Cloning, sequencing and fuctional study of gacA gene from Xanthomonas oryzae pv. oryzicola |
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| YANG Wan-feng,CHEN Lei,LIU Hong-xi,HU Bai-shi and LIU Feng-quan.Cloning, sequencing and fuctional study of gacA gene from Xanthomonas oryzae pv. oryzicola[J].Acta Microbiologica Sinica,2007,47(2):208-212. |
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| Authors: | YANG Wan-feng CHEN Lei LIU Hong-xi HU Bai-shi and LIU Feng-quan |
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| Institution: | Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China;Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China;Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China;Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China;Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Chinese Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095; China |
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| Abstract: | |
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| Keywords: | Xanthomonas oryzae pv oryzicola (Xooc) gacA gene disruption mutant chemotaxis |
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