| 苏云金芽胞杆菌YBT1520杀虫晶体蛋白基因的属性 |
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| 引用本文： | 孙明,刘子铎,喻子牛.苏云金芽胞杆菌YBT1520杀虫晶体蛋白基因的属性[J].微生物学报,2000,40(4):365-371. |
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| 作者姓名： | 孙明 刘子铎 喻子牛 |
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| 作者单位： | 华中农业大学生命科学技术学院农业部农业微生物重点实验室武汉 430070 |
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| 基金项目： | 国家863计划课题(101-03-01-01)资助 |
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| 摘 要： | 通过Ｓｏｕｔｈｅｒｎ杂交发现高毒力苏云金芽胞杆菌（Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ）ＴＢＴ－１５２０菌株含有两个杀虫晶体蛋白基因片段，其５’＝末端所在ＨｉｎｄⅢ片段分别为６．８ｋｂ和４．６ｋｂ，它们对应的基因分别命名为ｃｒｙ２１８和ｃｒｙ４．６。经ＰＣＲ鉴定，该菌含有ｃｒｙ１Ａａ、ｃｒｙ１Ａｂ和ｃｒｙ１Ａｃ基因，以及ｃｒｙ２基因，其中ｃｒｙ２１８属于ｃｒｙ１Ａｃ。分析了ｃｒｙ１Ａｃ基因
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| 关 键 词： | 苏云金芽胞杆菌 杀虫晶体蛋白基因 cry218基因 |
| 文章编号： | 0001-6209(2000)04-0365-07 |
CHARACTERIZATION OF THE INSECTICIDAL CRYSTAL PROTEIN GENES OF BACILLUS THURINGIENSIS YBT-1520 |
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| Sun Ming\ Liu Ziduo\ Yu Ziniu.CHARACTERIZATION OF THE INSECTICIDAL CRYSTAL PROTEIN GENES OF BACILLUS THURINGIENSIS YBT-1520[J].Acta Microbiologica Sinica,2000,40(4):365-371. |
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| Authors: | Sun Ming\ Liu Ziduo\ Yu Ziniu |
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| Institution: | College of Life Science and Technology, Huazhong Agricultural University, Key Laboratory of Agricultural Microbiology, Ministry of Agriculture, Wuhan 430070. |
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| Abstract: | The insecticidal crystal protein genes of highly toxic \?cillus thuringiensis\% YBT\|1520 were analyzed. Southern hybridization and PCR analysis indicated that this strain bears \%cry1Aa, cry1Ab,cry1Ac\% and \%cry2\% genes. The copy number of three \%cry1A\% genes is different and \%cry1Ac\% gene (here named \%cry218\%) is the highest. The restriction location of three genes is different from that in typical strains, such as HD 1 and HD 73 strains. 4190bp nucleotide sequence of \%cry218\% gene was determined and this gene was named \%cry1Ac10\% in the recently published gene nomenclature. |
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| Keywords: | Bacillus thuringiensis Insecticidal crystal protein gene Restriction location cry218\% gene |
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