| 建兰花叶病毒单克隆抗体的制备及检测应用 |
| |
| 引用本文： | 孟春梅,吴建祥,谢礼,郑锦凯,洪健.建兰花叶病毒单克隆抗体的制备及检测应用[J].微生物学报,2007,47(5):928-931. |
| |
| 作者姓名： | 孟春梅 吴建祥 谢礼 郑锦凯 洪健 |
| |
| 作者单位： | 1. 浙江大学生物技术研究所,杭州,310029
2. 浙江森禾种业股份有限公司,杭州,310020 |
| |
| 摘 要： | 用建兰花叶病毒(Cymbidium mosaic virus,CymMV)免疫的BALB/C鼠脾细胞与SP2/0鼠骨髓瘤细胞融合,经筛选克隆,获得3株能稳定传代并分泌抗CymMV单克隆抗体(McAb)的杂交瘤细胞(2C6、5B7和12G9),分别制备它们的单抗腹水。其中5B7和12G92株单克隆抗体腹水间接ELISA效价达10-6,3株单抗的抗体类型及亚类均为IgG1,轻链均为κ链。利用单克隆抗体建立了抗原包被间接ELISA(ACP-ELISA)检测CymMV的方法。蝴蝶兰病叶作1∶10240倍稀释、提纯CymMV病毒浓度为4.87ng/mL(每孔的病毒绝对量为0.487ng)时,该方法仍能检测到病毒。利用ACP-ELISA方法检测了田间样品,发现CymMV在兰花上发病很普遍。
|
| 关 键 词： | 建兰花叶病毒 单克隆抗体 |
| 文章编号： | 0001-6209（2007）05-0928-04 |
| 收稿时间： | 2007/5/16 0:00:00 |
| 修稿时间： | 8/8/2007 12:00:00 AM |
Production of monoclonal antibodies to Cymbidium mosaic virus and application in Orchids virus detection |
| |
| MENG Chun-mei,WU Jian-xiang,XIE Li,ZHENG Jin-kai and HONG Jian.Production of monoclonal antibodies to Cymbidium mosaic virus and application in Orchids virus detection[J].Acta Microbiologica Sinica,2007,47(5):928-931. |
| |
| Authors: | MENG Chun-mei WU Jian-xiang XIE Li ZHENG Jin-kai and HONG Jian |
| |
| Institution: | Institute of Biotechnology; Zhejiang University; Hangzhou 310029; China;Institute of Biotechnology; Zhejiang University; Hangzhou 310029; China;Institute of Biotechnology; Zhejiang University; Hangzhou 310029; China;Zhejiang Senhe Seed Co. Ltd.; Hangzhou 310020; China;Institute of Biotechnology; Zhejiang University; Hangzhou 310029; China |
| |
| Abstract: | Three hybridoma cell lines, 2C6, 5B7 and 12G9, secreting monoclonal antibodies (McAbs) against Cymbidium mosaic virus (CymMV) were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/ C immunized by the CymMV particles. The three McAbs could specifically react with CymMV. The titres of ascitic fluids of two McAbs are up to 10(-6) in I-ELISA. Isotypes and subclasses of the the three McAbs belong to IgG1. Isotypes of light strains of the three McAbs all belong to kappa. They were used in antigen-coated plate (ACP)-ELISA for CymMV detection, and ACP-ELISA could successfully detect 0.487 ng of purified CymMV or virus in plant sap diluted 1:10240. The presence of CymMV in field Orchids tissues was investigated with ACP-ELISA. |
| |
| Keywords: | ACP-ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
|
