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根癌土壤杆菌C58 Cereon中分泌蛋白信号肽分析
引用本文:范成明,李成云,赵明富,何月秋.根癌土壤杆菌C58 Cereon中分泌蛋白信号肽分析[J].微生物学报,2005,45(4):561-566.
作者姓名:范成明  李成云  赵明富  何月秋
作者单位:云南农业大学植物保护学院,昆明,650201
摘    要:利用SignalP3.0、LipoP1.0、TMHMM2.0和TargetP1.014种蛋白分析软件预测了Agrobacterium tumefaciens C58 Cereon菌株全部基因组的4554个ORF编码的蛋白信号肽,共发现203个信号肽,且它们的氨基酸残基相对保守。其中158条具分泌型信号肽,9条具RR-motif型信号肽,28条具信号肽酶Ⅱ型信号肽,8条具细菌素-信息素型信号肽,但只有分泌蛋白AGR-C-1878p和AGR-C-1880p的信号肽氨基酸残基完全相同,表明信号肽是高度变异的。

关 键 词:基因组,信号肽,蛋白分析
文章编号:0001-6209(2005)04-0561-06
修稿时间:2005年1月4日

Analysis of signal peptides of the secreted proteins in Agrobacterium tumefaciens C58
FAN Cheng-ming,LI Cheng-yun,ZHAO Ming-fu,HE Yue-qiu.Analysis of signal peptides of the secreted proteins in Agrobacterium tumefaciens C58[J].Acta Microbiologica Sinica,2005,45(4):561-566.
Authors:FAN Cheng-ming  LI Cheng-yun  ZHAO Ming-fu  HE Yue-qiu
Institution:Plant Protection College, Yunnan Agricultural University, Yunnan Agricultural University, Kunming 650201, China. fanchengming@126.com
Abstract:The 4554 ORFs of Agrobacterium tumefaciens C58 Cereon were used for the prediction of signal peptides by the network tools, such as SignalP3.0, LipoP1.0, TMHMM2.0 and TargetP1.01. Total 203 signal peptides with conserved amino residues are found, among them, 158 are secretary types, 9 are RR-motif types, 28 are SignalPase II types and 8 are bacteriocin-pheromone types. However, only two signal peptides from the secreted proteins, AGR-C-1878p and AGR-C-1880p have the same amino sequences, showing the signal peptides of the strain are highly variable.
Keywords:Genome  Signal peptides  Protein prediction  Secretome
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