| 真菌天然产物异源生产研究进展 |
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| 引用本文： | 马紫卉,李伟,尹文兵.真菌天然产物异源生产研究进展[J].微生物学报,2016,56(3):429-440. |
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| 作者姓名： | 马紫卉 李伟 尹文兵 |
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| 作者单位： | 中国科学院微生物研究所, 真菌学国家重点实验室, 北京 100101;中国科学院大学, 北京 100449,中国科学院微生物研究所, 真菌学国家重点实验室, 北京 100101,中国科学院微生物研究所, 真菌学国家重点实验室, 北京 100101 |
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| 基金项目： | 国家自然科学基金(31470178);中国科学院“百人计划”(Y454011001);北京市自然科学基金(5152018) |
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| 摘 要： | 真菌天然产物是天然药物的重要来源之一,大规模真菌基因组序列测序的完成表明真菌具有产生丰富的次级代谢产物的潜能。然而,许多真菌或生长缓慢,或不适宜在实验室条件下培养,或难以进行遗传操作,或化合物产量极低等,这些因素导致大量有价值的真菌天然产物无法获得。利用异源表达系统对真菌天然产物进行生产是发现新天然产物及解析其生物合成途径的有效手段,并为定向的以合成生物学的手段去合成重要活性分子奠定基础。本文对目前用于真菌天然产物生产的各种异源表达系统进行了综述,并结合最新的DNA组装技术展望了异源表达系统在真菌天然产物研究中的应用价值和前景。
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| 关 键 词： | 天然产物 基因簇 异源表达 合成生物学 |
| 收稿时间： | 2015/9/19 0:00:00 |
| 修稿时间： | 2015/12/5 0:00:00 |
Progress in heterologous expression of fungal natural products-A review |
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| Zihui M,Wei Li and Wenbing Yin.Progress in heterologous expression of fungal natural products-A review[J].Acta Microbiologica Sinica,2016,56(3):429-440. |
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| Authors: | Zihui M Wei Li and Wenbing Yin |
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| Institution: | State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100449, China,State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China and State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Fungal products are important resources for natural drugs. Availabilities of many fungal genome sequences indicate that fungi have the huge potential to produce secondary metabolites. However, many valuable fungal natural products are hard to obtain under regular lab conditions because fungi are slow to grow, difficult to genetically modify, or have low yield of desired metabolites. Therefore, developing a heterologous expression system has been an efficient approach to discover novel natural product and elucidate the complicated biosynthetic pathways. This review describes several heterologous expression systems that have been successfully used for the expression of fungal biosynthetic gene clusters. Also, we address the application perspective based on the emerging DNA assembly technologies. |
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| Keywords: | natural product gene cluster heterologous expression synthetic biology |
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