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丝状真菌高效表达异源蛋白研究进展
引用本文:钟耀华,王晓利,汪天虹.丝状真菌高效表达异源蛋白研究进展[J].微生物学报,2008,24(4):531-540.
作者姓名:钟耀华  王晓利  汪天虹
作者单位:山东大学微生物技术国家重点实验室, 济南 250100;山东大学微生物技术国家重点实验室, 济南 250100;山东大学微生物技术国家重点实验室, 济南 250100
基金项目:国家自然科学基金(Nos.30670029, 30270024)、国家重点基础研究规划(973) (No. 2003CB716006) 和教育部博士点基金(No. 20040422042)资助。
摘    要:丝状真菌是具有高效分泌蛋白质潜力的真核表达系统, 能对蛋白质进行翻译后修饰, 如蛋白质糖基化等; 并且比植物、昆虫和哺乳动物细胞具有更快的生长速率。近年来, 随着真菌分子遗传技术和菌种改良策略的进步, 尤其是真菌基因组学的发展, 利用丝状真菌生产异源蛋白越来越受到关注。综述了丝状真菌作为细胞工厂生产异源蛋白的最新探索与进展, 其中包括功能基因组学在蛋白表达与分泌研究中的应用, 同时探讨了异源蛋白表达和生产的改进策略。

关 键 词:丝状真菌    异源蛋白表达    蛋白质分泌    蛋白质糖基化    功能基因组学

Recent Advances in the Production of Heterologous Proteins in Filamentous Fungi
Yaohua Zhong,Xiaoli Wang and Tianhong Wang.Recent Advances in the Production of Heterologous Proteins in Filamentous Fungi[J].Acta Microbiologica Sinica,2008,24(4):531-540.
Authors:Yaohua Zhong  Xiaoli Wang and Tianhong Wang
Institution:State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
Abstract:Filamentous fungi can secrete large amounts of proteins, glycosylate proteins and grow faster than plant, insect or mammalian cells. With the advances in fungal molecular genetics, strain improvement, and especially fungal genomics, filamentous fungi are developed as microbial cell factories for the production of heterologous proteins. This review focuses on recent developments of filamentous fungi as production hosts, such as protein quality control mechanisms, the secretion pathways, protein modification, strain stability, and most importantly the application of functional genomics in protein expression. At the same time, the strategies for improving heterologous protein production were also discussed in details.
Keywords:filamentous fungi  heterologous protein expression  protein secretion  protein glycosylation  fungal genomics
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