| 柳树黄化病植原体的分子分类 |
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| 引用本文： | 魏婷,吴云锋,侯伟,武科科,李毅然,张珏,孙润红.柳树黄化病植原体的分子分类[J].微生物学报,2009,49(3):389-394. |
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| 作者姓名： | 魏婷 吴云锋 侯伟 武科科 李毅然 张珏 孙润红 |
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| 作者单位： | 西北农林科技大学植保学院,陕西省农业分子生物学重点实验室,植保资源与病虫害治理教育部重点实验室,杨凌,712100 |
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| 基金项目： | 国家自然科学基金，教育部长江学者和创新团队发展计划，高等学校创新引智计划 |
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| 摘 要： | 目的]柳树黄化病是一种重要的植原体病害,本研究旨在明确柳树黄化病植原体(Willow Yellow phytoplasma,WY)的分类地位,为进一步开展致病性和防治研究奠定基础.方法]采用植原体特异引物通过PCR方法从患病植株DNA中扩增植原体16S rDNA基因和核糖体蛋白基因(ribosomal proteins gene,rp),对所得的序列进行分析,构建同源进化树,并用限制性片段长度多态性(RFTJP)对巢式PCR产物进行分析.结果]首次从柳树黄化病植原体中分离出了16S rDNA基因和rp基因,大小分别为1246 bp和1212 bp.通过对植原体16S rDNA和rp基因的核苷酸同源性比较和RFLP分析,发现该分离物与16S rI组的核苷酸同源性均在99%以上,与16S rI-C亚组中的小麦蓝矮病植原体同源性高达99.8%(16Sr DNA)和99.6%(rp),且RFLP分析与16SrI-C亚组的植原体有相同的酶切条带.结论]柳树黄化植原体应划分于16SrI-C亚组.
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| 关 键 词： | 柳树黄化 植原体分类 RFLP分析 核糖体蛋白 巢式PCR |
| 收稿时间： | 2008/9/30 0:00:00 |
| 修稿时间： | 2008/11/21 0:00:00 |
Molecular Classification of a Phytoplasma Associated with Willow Yellow Disease |
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| Ting Wei,Yunfeng Wu,Wei Hou,Keke Wu,Yiran Li,Jue Zhang and Runhong Sun.Molecular Classification of a Phytoplasma Associated with Willow Yellow Disease[J].Acta Microbiologica Sinica,2009,49(3):389-394. |
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| Authors: | Ting Wei Yunfeng Wu Wei Hou Keke Wu Yiran Li Jue Zhang and Runhong Sun |
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| Institution: | College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest Sci-Tech University of Agriculture and Forestry,Yangling 7121 |
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| Abstract: | |
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| Keywords: | 16s rDNA |
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