损伤条件下油菜黄单胞菜豆致病变种内源过氧化氢与间体形成的联系

【目的】本实验通过透射电子显微镜观察黄单胞菌在细胞损伤条件下的亚细胞结构和过氧化氢积累定位的变化。【方法】采用氯化铈对过氧化氢特异染色的组织化学法。【结果】细菌细胞受损伤后,出现了一个细胞壁之外的过氧化氢大量积累的额外位点。并且这个额外位点出现的频率和过氧化氢积累量都与细胞损伤的程度密切相关。另一方面,亚细胞结构的常规染色结果也显示,受到损伤的细胞中也出现一个额外的亚显微结构,即间体。间体出现的频率和大小也随着细胞损伤程度的增加而显著上升。【结论】多元线性回归分析的结果证明细胞损伤条件下细菌中出现的额外过氧化氢大量积累的位点就是间体。细胞损伤后间体中的过氧化氢积累对受损细胞应是一种主动调控机制。

Correlation between endogenous hydrogen peroxide and mesosome formation in cellular injured bacteria

Abstract: Objective] Ultrastructural alteration and hydrogen peroxide (H2O2) localization were examined in Xanthomonas under cellular injury using transmission electron microscopy. Methods] Histochemical methods were used in the present study. Results] Intriguingly, the injury led to presence of an additional location of H2O2 accumulation within the cells. There was an association between the frequency and size of the additional location of H2O2 accumulation and the degrees of injury. Furthermore, an additional ultrastructure, mesosomes, was also present in injured cells. The frequency and size of mesosomes also increased with the increasing degrees of injury. Conclusion] Result of multiple linear regression showed that the size of mesosome plays as a key factor in the quantity of excess H2O2 accumulation in bacteria under cellular injury. Linear correlation was confirmed between quantity of excess H2O2 accumulation and the size of mesosome in injured cells. This finding intensely indicated that mesosomes are just the additional location of H2O2 accumulation in cells under cellular injury. The excess H2O2 accumulation in mesosomes should be a positive regulatory mechanism in bacteria under cellular injury.