酵母超氧物歧化酶高产菌的选育

采用常规筛选方法从300多株不同种属的酵母菌中筛选到两株细胞生物量和超氧物歧化酶(SOD)含量都较高的菌株(酿酒酵母,编号为Y-8和Y一111)作为实验出发菌.经单倍体分离、N-甲基-N-亚硝基-N’-硝基胍(MNNG)诱变和群体杂交等手段,从中选育出一株细胞生物量略高于实验出发菌、超氧物歧化酶高达1350U/g湿菌体的SOD高产菌株(编号为ZDF-48),它的SOD产量分别为实验出发菌株Y-8和Y-111的2.2 倍和2.4倍.经分离纯化后蛋白含量及超氧物歧化酶活性测定等研究,证明我们选育出的ZDF-48是一株生产超氧物歧化酶的优良品系.

THE BREEDING OF THE SOD HIGH-PRODUCING STRAIN FROM YEASTS

The SOD producing strains were obtained from more than 300 strains of different species and genera of yeasts. A SOD high-producing hybrid (ZDF-48) was bred through isolation of haploid, mutation by MNNG and population hybridization. The biomass of the hybrid was the same as original strains and the content of SOD reached 1350U/g fresh cells, which were 2. 2 and 2. 4 times of original strains' (Saccharomyces cerevisiae Y-8 and Y-111) respectively. The isolation and purification of SOD, protein concentration and bio-activity have been also studied. We concluded that ZDF-48 belonged to an excellent SOD producer.