黄孢原毛平革菌合成木素过氧化物酶的营养调控

本文研究了营养条件对黄孢原毛平革茵(Phanerochale chrysosporium)ME-116合成木素过氧化物酶及其同工酶组分的影响.在最适培养条件下获得1500U/L的酶活.高效液相色谱分离的5个同工酶组分中以P_2组分含量最高.低碳高氮培养基最适于酶的合成.降低氮和KH_2PO_4含量致使各组分含量下降,而改变MgSO_4和CaCl_2浓度对P_2组分无影响.表面活性剂吐温80主要通过提高细胞膜透性而增加酶的合成.黎芦醇对5种同工酶组分的合成均有诱导作用.培养基中各营养因子对木素过氧化物酶的合成存在着复杂的交互作用.

NUTRITIONAL REGULATION OF SYNTHESIS OF LIGNIN PEROXIDASE BY PHANEROCHATE CHRYSOSPORIUM ME-446

The effects of nutritional conditions on synthesis of lignin peroxidase and its isoenzymes by Phanerochate chrysosporium ME-446 were studied. Under the optimal culture conditions, the activity of 1 500U/L was obtained. Of the five isoenzymes separated with high performance liquid chromatergraphy,P2 content is the highest. Low carbon and high nitrogen medium is most suitable for synthesis of the enzyme. Lower contents of nitrogen or KH2PC)4 make decrease of all iosenzymes, while changing concentrations of MgSO4 or CaCl2 has no effects on P2 component. Tween80 mainly increases permeability of cell membrane. Veratrylalcohol induces synthesis of each of lignin peroxidase isoenzymes. There exist complicated mutual functions between nutritional factors in culture on the enzyme synthesis.