| 3-氰基吡啶水合酶产生菌的筛选及其酶形成条件北大核心 |
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| 引用本文： | 赵爱民,李文忠.3-氰基吡啶水合酶产生菌的筛选及其酶形成条件北大核心[J].微生物学报,1994,34(2):131-136. |
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| 作者姓名： | 赵爱民 李文忠 |
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| 作者单位： | 中国科学院微生物研究所 北京100080
(赵爱民，李文忠)，中国科学院微生物研究所 北京100080(杨惠芳) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 应用富集培养和梯度底物浓度定向筛选技术,从长期被腈化物污染的土壤中筛选到一株产 3-氰基吡啶水合酶(3-cyanopyridine hydratase)活性较高的马红球菌(Rhodococcus e-qui)SHB-121.研究了该菌3-氰基吡啶水合酶的最适形成条件.在最适条件下,酶的比活力达5.3u/mg干细胞,比在初筛条件下的酶活力提高95倍,而在其细胞内共存的尼克酰胺(烟酰胺)水解酶活力很低.
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| 关 键 词： | 微生物 马红球菌 氰基吡啶 水合酶 |
SCREENING OF 3-CYANOPYRIDINE HYDRATASE-PRODUCING STRAINS AND FORMATION OF THE ENZYME |
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| Zhao Aimin Li Wenzhong Yang Huifang.SCREENING OF 3-CYANOPYRIDINE HYDRATASE-PRODUCING STRAINS AND FORMATION OF THE ENZYME[J].Acta Microbiologica Sinica,1994,34(2):131-136. |
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| Authors: | Zhao Aimin Li Wenzhong Yang Huifang |
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| Abstract: | A bacterium, Rhodococcus equi SHB-121 forming 3-cyanopyridine hydratase was screened from soil polluted by nitriles according to the biomass and the enzyme activity. Its activity was the highest among 200 nitrile-utilizing bacteria isolated. The optimum formation conditions for the 3-cyanopyridine hydratase of SHB-121 have been studied. Under the optimum conditions ,5. 3 u/mg dry cell weight of specific activity was produced , and the coexistent amidase activity was very low. The specific activity of strain SHB-121 was about 95 times higher than that in primitive screening medium. |
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| Keywords: | Microbial conversion Rhodococcus equi 3-cyanopyridine hydratase Nicoti-namide |
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