| 猪圆环病毒2型与猪细小病毒共感染对猪肺泡巨噬细胞吞噬功能及其干扰素表达水平的影响 |
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| 引用本文： | 刘祥义,陈立功,宋勤叶,杨芳,李艳琴,左玉柱,焦健达,王秀萍.猪圆环病毒2型与猪细小病毒共感染对猪肺泡巨噬细胞吞噬功能及其干扰素表达水平的影响[J].微生物学报,2011,51(1):105-114. |
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| 作者姓名： | 刘祥义 陈立功 宋勤叶 杨芳 李艳琴 左玉柱 焦健达 王秀萍 |
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| 作者单位： | 河北农业大学动物科技学院,保定,071001 |
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| 基金项目： | 国家自然科学基金项目（30771600） |
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| 摘 要： | 【目的】分析猪圆环病毒2型(PCV2)与猪细小病毒(PPV)共感染对猪肺泡巨噬细胞(PAM)吞噬功能及其干扰素表达水平的影响,为进一步阐明猪断奶后多系统衰减综合征的发病机制提供实验依据。【方法】将48头5周龄健康仔猪随机分为PCV2组、PPV组、PCV2/PPV组和对照组,每组12头。PCV2和PPV组经口鼻途径分别接种PCV2或PPV,PCV2/PPV组同时接种PCV2和PPV,对照组接种细胞营养液。感染后3、7、14和35d(dpi)从每组随机选择3头剖杀,检测PAM的存活率、吞噬活性及其α1型干扰素(IFN-α1)、γ干扰素(IFN-γ)mRNA的表达水平。【结果】PCV2组和PCV2/PPV组PAM的存活率于3、7、14dpi均低于对照组,35dpi均与对照组PAM的存活率接近,PCV2与PCV2/PPV两组之间差异不显著(P>0.05);3个病毒感染组PAM的吞噬功能均显著下降(P<0.01),其中PCV2/PPV组的吞噬功能低于PCV2组(P<0.05);PCV2/PPV组PAM中IFN-α1和IFN-γ mRNA的水平持续低于PCV2、PPV组和对照组的水平(P<0.01)。【结论】PCV2与PPV共感染没有引起PAM的活力进一步下降,但导致其吞噬功能及IFN-α1和IFN-γmRNA的表达水平持续降低。
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| 关 键 词： | PCV2 PPV 共感染 猪肺泡巨噬细胞 吞噬功能 干扰素 猪 |
| 收稿时间： | 2010/6/26 0:00:00 |
| 修稿时间： | 9/3/2010 12:00:00 AM |
Coinfection effects of porcine circovirus type 2 and porcine parvovirus in vivo on phagocytosis and interferon mRNA expression of porcine alveolar macrophages |
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| Xiangyi Liu,Ligong Chen,Qinye Song,Fang Yang,Yanqin Li,Yuzhu Zuo,Jianda Jiao and Xiupin Wang.Coinfection effects of porcine circovirus type 2 and porcine parvovirus in vivo on phagocytosis and interferon mRNA expression of porcine alveolar macrophages[J].Acta Microbiologica Sinica,2011,51(1):105-114. |
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| Authors: | Xiangyi Liu Ligong Chen Qinye Song Fang Yang Yanqin Li Yuzhu Zuo Jianda Jiao and Xiupin Wang |
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| Institution: | College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China;College of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China |
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| Abstract: | Objective]Our study is to analyse the coinfection effects of porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) in vivo on phagocytosis and interferon mRNA expression level of porcine alveolar macrophages (PAM).Methods]Forty-eight 5-week-old healthy piglets were divided randomly into 4 groups of 12 (PCV2,PPV,PCV2 /PPV and the control groups).The piglets in PCV2 group were inoculated oronasally with 3 mL of porcine circovirus type2 (PCV2,105.61 TCID50 /0.1 mL),PPV group with 3 mL of porcine parv... |
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| Keywords: | Keywords: PCV2 PPV co-infection PAM phagocytosis interferon swine |
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