谷胱甘肽对铜绿假单胞菌exoS 和exoY基因的影响

摘要: 【目的】研究谷胱甘肽对铜绿假单胞菌exoS和exoY基因表达的影响。【方法】利用丁硫氨酸亚砜胺和马来酸二乙酯同时耗竭细胞内的谷胱甘肽，并构建包含被lacZGm破坏的谷胱甘肽合成酶基因的突变体。通过分别连有exoS 和exoY基因启动子的pMS402质粒上Lux报道子发光值大小检测exoS 和exoY基因表达变化情况。【结果】exoS和exoY基因的表达在用化学药品耗竭的细胞中或是在谷胱甘肽合成酶突变体中都降低。【结论】铜绿假单胞菌细胞内的谷胱甘肽可以促进exoS和exoY的表达。这将为进一步研究铜绿假单胞菌的感染以及致病性机理提供一定的理论基础。

Impact of glutathione on the gene expression of exoY and exoS in Pseudomonas aeruginosa

Abstract: Objective]: To study the impact of GSH (glutathione) on the gene expression of exoY and exoS in Pseudomonas aeruginosa. Methods]: We treated P. aeruginosa with BSO (buthionine sulfoximine) and DEM (diethylmaleate) to deplete GSH, or construct the P. aeruginosa mutant containing a lacZGm disrupted gshB (glutathione synthetase) gene by homologous recombination technology. The expression of exoY and exoS was determined by measuring light production of the lux-based reporters on pMS402. Results]: The expression of exoY and exoS decreased in the gshB mutant and P. aeruginosa treated with BSO and DEM. Conclusion]: GSH in the P. aeruginosa can increase the expression of the genes exoY and exoS. Furthermore, this result provided possibilities to elucidate the molecular mechanisms of pathogenesis and immune response triggered by P. aeruginosa.