柠檬醛对黄曲霉质膜损伤机制的初步研究

与正常生长的黄曲霉对照 ,通过测定经柠檬醛毒化的菌丝体对还原糖和蛋白质利用率、Na+ ,K+ ] ATPase分解ATP活性、细胞电解质渗出率、并结合扫描电镜和快速显微多道分光光度法观察菌丝体细胞及孢子形态变化 ,结果表明经该醛MIC毒化后 ,菌丝体细胞及孢子表面疏松而粗糙 ;隘痕缩小并关闭 ;电导率增加 5 2 8% ;对还原糖和蛋白质的利用速率分别下降 6 1 5 %和 44 3 % ;孢子萌发率下降至 6 1 4% ;该醛能明显改变细胞质膜的分子结构 ,使其失去选择通透性而抑制菌丝体生长和孢子萌发率。

PRELIMINARY STUDY ON CITRAL IMPAIRES THE ASPERGILLUS FLA VUS MEMBRANE

Compared with the normally growing A.flauas, the content as below was determined: the utilization ratio to protein and reducing sugar of hyphostroma poisened by citral, the activity of Na ,K ]-ATPase capable of decomposition ATP, and the seepagevity ratio of electrolyte. In addition, the shape change in spore was observed via the scanning electron microscope (SEM) and the fast multi-channel micro-spectrophotomer (FMCM).The result above all suggested facts as following after it's poised by the citral in MIC.The surface of hyphostroma and spore turned into be porous and rough. The pass trace on spore shriveled and closed. The rate of conduct electricity increased by 52.8%. The utilization ratio to protein and reducing sugar respectively decreased 61.5% and 44.3%. The rate of spore's sprout dropped to 61.4%. The molecular structure of membrane was so distinctly changed that it lost the selective permeability. There was inhibition on hyphostroma growth and spore sprout.