| 真养雷氏菌DKC1菌株镉抗性czcC基因的克隆与表达 |
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| 引用本文： | 段学军,闵航,吕文平,夏颖.真养雷氏菌DKC1菌株镉抗性czcC基因的克隆与表达[J].微生物学报,2004,44(4):461-464. |
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| 作者姓名： | 段学军 闵航 吕文平 夏颖 |
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| 作者单位： | 1. 浙江大学生命科学学院,杭州,310029;中原工学院,郑州,450009
2. 浙江大学生命科学学院,杭州,310029 |
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| 基金项目： | 国家科技部社会公益研究专项资金项目(177-2-3) |
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| 摘 要： | 利用PCR技术从真养雷氏菌 (Ralstoniaeutropha)菌株质粒中扩增出 1 2kb的镉抗性系统结构修饰蛋白的编码基因czcC ,然后将其克隆到pGEM T easy载体上 ,构建重组质粒 ,经EcoRⅠ酶切分析和核苷酸序列分析 ,与Gen Bank中登录的czcC基因序列相似性高达 98% ,显示其具有正确的czcC基因核苷酸序列 ,并利用pET 30a( )Vector在E .coliBL2 1中进行了成功表达。为进一步研究微生物抗镉机理及构建耐镉基因工程菌提供重要的基础资料
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| 关 键 词： | 真养雷氏菌 czcC 克隆与表达 |
| 文章编号： | 0001-6209(2004)04-0461-04 |
| 修稿时间： | 2003年11月24 |
Cloning and Expression of The Cadmium Resistant Determinant czcC in Escherichia coli |
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| DUAN Xue Jun , MIN Hang LU Wen Ping XIA Ying.Cloning and Expression of The Cadmium Resistant Determinant czcC in Escherichia coli[J].Acta Microbiologica Sinica,2004,44(4):461-464. |
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| Authors: | DUAN Xue Jun MIN Hang LU Wen Ping XIA Ying |
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| Institution: | DUAN Xue Jun 1,2 MIN Hang 1* LU Wen Ping 1 XIA Ying 1 |
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| Abstract: | The full length czcC gene encoding the structure modified protein in cadmium resistance system of Ralstonia eutropha was amplified by PCR and cloned into prokaryotic pGEM T easy,the recombinant plasmid was constructed. The sequencing results showed that there was the comparability about 98% between the sequence from the strain and the same gene accessed on the GenBank database. Then, it was successfully expressed in the E.coli BL21. The work will be available in further studies on cadmium resistance of microorganism and the design of gene engineering bacteria which are able to live in environments contaminated with higher concentration of cadmium. |
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| Keywords: | Ralstonia eutropha czcC Cloning and Expression |
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