| 扁绒泡菌Physarum compressum原质团细胞核核骨架制备技术的研究 |
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| 引用本文： | 王晓丽,李玉.扁绒泡菌Physarum compressum原质团细胞核核骨架制备技术的研究[J].微生物学报,2008,27(2):177-182. |
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| 作者姓名： | 王晓丽 李玉 |
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| 作者单位： | 吉林农业大学菌物研究所 长春 130118;吉林农业大学菌物研究所 长春 130118 |
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| 基金项目： | 国家自然科学基金项目（No. 30070624）；吉林农业大学博士启动基金（No. 203136） |
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| 摘 要： | 以扁绒泡菌显型原质团为材料，进行细胞核的提纯、核骨架的制备及电镜观察。结果表明：用2mol/L NaCl+TritonX-100/NP40可得到具有复合纤维的核骨架，而用Lis + TritonX-100/NP40得到的核骨架具有核纤层，不使用RNase得到了具有网状的细胞核骨架，RNA在核骨架的结构形态中起重要作用。阐述了原质团中游离细胞核在细胞生物学研究中的意义。
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| 关 键 词： | 黏菌，营养生长阶段，细胞器，核基质 |
| 收稿时间： | 5/8/2007 12:00:00 AM |
Nuclear matrix of Physarum compressum phanero-plasmodium |
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| WANG Xiao-Li and LI Yu.Nuclear matrix of Physarum compressum phanero-plasmodium[J].Acta Microbiologica Sinica,2008,27(2):177-182. |
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| Authors: | WANG Xiao-Li and LI Yu |
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| Institution: | Institute of Mycology, Jilin Agricultural University, Changchun 130118, China;Institute of Mycology, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Nuclear matrix prepared from phaneroplasmodium of Physarum compressum was observed under TEM. The nuclear matrix with compound-fibres was obtained by use of 2mol/L NaCl + TritonX-100/NP40, while nuclear matrix with nuclear lamina was obtained by using Lis + TritonX-100/NP40. Net nuclear matrix was obtained without using RNase, showing RNA played an important role in nuclear matrix structure. The significance of dissociated nuclei of plasmodium in the study of cell biology was expounded in this paper. |
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| Keywords: | Myxomycetes vegetative growth phase organelle cellular skeleton |
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