酵母提取物和海带水提液促大肠杆菌生长机理初步研究 |
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引用本文: | 李君文,孙薇,王新为,宋农,金敏.酵母提取物和海带水提液促大肠杆菌生长机理初步研究[J].微生物学报,2004,44(5):695-697. |
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作者姓名: | 李君文 孙薇 王新为 宋农 金敏 |
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作者单位: | 军事医学科学院卫生学环境医学研究所,天津,300050 |
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基金项目: | 国家"863计划"(2002AA601240);天津市科技攻关重大项目(0231807111) |
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摘 要: | 研究海带水提液和酵母提取物促大肠杆菌生长的机理。采用二维电泳 (2 DE)和生物质谱等蛋白质组技术对细菌的全蛋白质表达进行研究。发现 8种蛋白出现有规律的变化 ,其中 ,2种蛋白在对数期各组表达量均增高 ,4种蛋白在各组表达量下降且实验组下降更明显 ,1种蛋白在实验组表达量明显降低而对照组无明显变化。
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关 键 词: | 酵母提取物 海带水提液 大肠杆菌 促生长 机理 |
文章编号: | 0001-6209(2004)05-0695-03 |
修稿时间: | 2003年12月23 |
Study on Mechanisms of Escherichia coli Growth Stimulated by Yeast Extract and Echlonia Kurome Okum Water Extract |
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Abstract: | To elucidate the mechanisms of yeast extraction and Echlonia Kurome Okum water extraction to stimulate the growth of Escherichia coli. Two-dimensional electrophoresis (2-DE) and bio-mass-spectrum were used to observe the changes of whole-protein expression caused by certain stimulants. It was found that the expression of 8 proteins changed in different ways. Two proteins leveled up but failed to be identified; 4 proteins declined much more in experimental groups than in control. There are some proteins participating to regulate growth of Escherichia coli. |
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Keywords: | Yeast extraction Echlonia Kurome Okum water extraction Escherichia coli Growth Mechanism |
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