| 苏云金芽胞杆菌等离子复合体诱变提高抗鳞翅目害虫的杀虫毒力 |
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| 引用本文： | 彭金莉,朱梓榕,陈文慧,罗斯思,周朋吉,全梅芳,孙运军,夏立秋,丁学知.苏云金芽胞杆菌等离子复合体诱变提高抗鳞翅目害虫的杀虫毒力[J].微生物学报,2022,62(4):1390-1400. |
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| 作者姓名： | 彭金莉 朱梓榕 陈文慧 罗斯思 周朋吉 全梅芳 孙运军 夏立秋 丁学知 |
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| 作者单位： | 湖南师范大学生命科学学院, 省部共建淡水鱼类发育生物学国家重点实验室, 微生物分子生物学湖南省重点实验室, 湖南 长沙 410081 |
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| 基金项目： | 国家重点研发计划(2017YFD0201201);国家自然科学基金(31370116) |
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| 摘 要： | 目的]苏云金芽胞杆菌(Bacillus thuringiensis,Bt)D18对鳞翅目、鞘翅目等农业害虫具有杀虫毒力,本研究拟通过复合诱变育种,筛选出杀虫毒力更高的突变菌.方法]经四轮室温常压等离子体(ARTP)诱变和一轮ARTP-NTG复合诱变后,镜检形态观察与生物毒力测定筛选高毒力菌株,SDS-PAGE检测C...
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| 关 键 词： | 苏云金芽胞杆菌 杀虫晶体蛋白 室温常压等离子体 复合诱变 |
| 收稿时间： | 2021/7/15 0:00:00 |
| 修稿时间： | 2021/9/18 0:00:00 |
Improvement of the virulence of Bacillus thuringiensis to lepidopteran pests by ARTP and NTG mutagenesis |
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| PENG Jinli,ZHU Zirong,CHEN Wenhui,LUO Sisi,ZHOU Pengji,QUAN Meifang,SUN Yunjun,XIA Liqiu,DING Xuezhi.Improvement of the virulence of Bacillus thuringiensis to lepidopteran pests by ARTP and NTG mutagenesis[J].Acta Microbiologica Sinica,2022,62(4):1390-1400. |
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| Authors: | PENG Jinli ZHU Zirong CHEN Wenhui LUO Sisi ZHOU Pengji QUAN Meifang SUN Yunjun XIA Liqiu DING Xuezhi |
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| Institution: | Hunan Provincial Key Laboratory of Microbial Molecular Biology, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China |
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| Abstract: | Objective] Bacillus thuringiensis(Bt) D18 is virulent to agricultural pests of Lepidoptera and Coleoptera.This study aims to improve the virulence of D18 by compound mutagenesis.Methods] After four rounds of atmospheric and room temperature plasma (ARTP) mutagenesis and one round of ARTP-N-methyl-N''-nitro-N-nitrosoguanidine (ARTP-NTG) mutagenesis,highly virulent mutants were screened out by microscopy and virulence bioassay.To reveal the reason for the increase of virulence,we detected the Cry proteins by SDS-PAGE and key cry genes by qRT-PCR.Results] Two highly virulent mutants,An-L5-1 and An-L5-7 were screened out,compared with the wild strain D18,An-L5-1 and An-L5-7 demonstrated slightly early decline,improved ability of sporulation,80.47% and 88.31% rise of Cry protein expression,and significantly stronger virulence to Plutella xylostella and Mythimna seperata.Conclusion] The increase in the virulence of An-L5-1 and An-L5-7 was mainly attributed to the significantly up-regulated expression of cry1Aa,cry2Aa,and sigK. |
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| Keywords: | Bacillus thuringiensis insecitcidal crystal protein ARTP compound mutagenisis |
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