| 检测心肌细胞钾离子通道蛋白活化水平方法的优化 |
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| 引用本文： | 王曦,张磊,周士胜,邹伟.检测心肌细胞钾离子通道蛋白活化水平方法的优化[J].微生物学报,2008,24(3):521-524. |
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| 作者姓名： | 王曦 张磊 周士胜 邹伟 |
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| 作者单位： | 辽宁师范大学生命科学学院, 大连 116029;辽宁师范大学生命科学学院, 大连 116029;大连大学医学部, 大连 116622;辽宁师范大学生命科学学院, 大连 116029 |
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| 基金项目： | 国家自然科学基金资助 (No. 30570665)。 |
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| 摘 要： | 介绍了一种如何合理的利用蛋白质免疫沉淀和蛋白质免疫印迹相结合的方法检测大鼠心肌细胞钾离子通道蛋白Kv1.2和Kv1.5的表达与活化水平。实验结果表明, 与单独利用免疫印迹的方法相比较, 本实验是对钾离子通道蛋白及其它亚家族的钾通道蛋白磷酸化表达水平检测方法的一种优化, 从而获得一套可行、简单、合理的实验方案, 同时也提高了检测的准确性, 敏感性及特异性。
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| 关 键 词： | 蛋白质免疫沉淀 大鼠心肌细胞 钾离子通道蛋白 |
| 收稿时间： | 7/5/2007 12:00:00 AM |
Optimization of Method for Detecting the Activation of Potassium Channels in Rat Cardiac Muscle Cells |
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| Xi Wang,Lei Zhang,Shisheng Zhou and Wei Zou.Optimization of Method for Detecting the Activation of Potassium Channels in Rat Cardiac Muscle Cells[J].Acta Microbiologica Sinica,2008,24(3):521-524. |
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| Authors: | Xi Wang Lei Zhang Shisheng Zhou and Wei Zou |
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| Institution: | Department of Life Sciences, Liaoning Normal University, Dalian 116029, China;Department of Life Sciences, Liaoning Normal University, Dalian 116029, China;Department of Medicine, Dalian University, Dalian 116622, China;Department of Life Sciences, Liaoning Normal University, Dalian 116029, China |
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| Abstract: | To detect the phosphorylation of potassium channel protein (Kv1.2 and Kv1.5) in rat cardiac muscle cells accurately, we applied the combined method of immunoprecipitation and Western blot in this study. Compared with using Western blot alone, the combination of immunoprecipitation and Western blot displayed high sensitivity to detect the activation of potassium channel proteins. Because of its simplicity, quickness and reproducibility, we find that this method was promising for detecting the phosphorylation of Kv1.2 and Kv1.5 proteins or other potassium channel proteins in rat cardiac muscle cells. |
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| Keywords: | immunoprecipitation rats cardiac muscle cells potassium channel proteins |
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