| 乳酸乳球菌乳脂亚种W56中质粒pJW566的生物学特性 |
| |
| 引用本文： | 孔健,顾笑梅,马桂荣.乳酸乳球菌乳脂亚种W56中质粒pJW566的生物学特性[J].微生物学报,2001,41(5):542-547. |
| |
| 作者姓名： | 孔健 顾笑梅 马桂荣 |
| |
| 作者单位： | 山东大学微生物技术国家重点实验室 |
| |
| 摘 要： | 从丹麦乳酪发酵启子乳酸乳球菌乳脂亚种 (Lactococcuslactissubsp .cremoris)W56中 ,分离到一个 2 2 4kb的质粒pJW566,将该质粒转化到无质粒且噬菌体敏感的L .lactisMG1 61 4、SMQ86菌株中 ,所得转化子对常见 963、c2和P335属的噬菌体具有一定抗性。经测定噬菌体以及含有pJW566的菌株所繁育的噬菌体效价 ,发现该质粒对外源DNA具有限制和修饰 (Re strictionandModification ,R M)作用。将pJW566转化到一株噬菌体敏感的乳酪工业生产菌株L .lactisCHCC2 2 81 ,在牛奶发酵中 ,表现出较强的噬菌体抗性。体外内切酶活性测定表明 ,该质粒具有的限制性内切酶需要Mg2 +和ATP ,而AdoMet(S adenosylmethionine,AdoMet)对酶活有促进作用
|
| 关 键 词： | 乳酸乳球菌 噬菌体 限制和修饰作用 质粒pJW566 |
| 文章编号： | 0001-6209(2001)05-0542-06 |
THE CHARACTERIZATION OF pJW566 FROM L. LACTIS SUBSP. CREMORIS W56 |
| |
| Kong Jian\ Gu Xiaomei\ Ma Guirong.THE CHARACTERIZATION OF pJW566 FROM L. LACTIS SUBSP. CREMORIS W56[J].Acta Microbiologica Sinica,2001,41(5):542-547. |
| |
| Authors: | Kong Jian\ Gu Xiaomei\ Ma Guirong |
| |
| Abstract: | The plasmid pJW566 was isolated from L.lactis subsp. cremoris W56,one strain for Danish chadder mixed starter cultures.The strain containing plasmid pJW566 showed resistance against three common phages species 936,c2 and P335 worldwide.It was found that pJW566 encoded for an restriction and modification system, and showed strong resistance to phage CHCP412 when it was introduced into the industrial strain L.lactis CHCC2281 in milk medium.The endonuclease activity analysis indicated that the endonuclease required Mg 2 ,ATP, and was stimulated by AdoMet. |
| |
| Keywords: | Lactococcus lactis Bacteriophage Restriction and modification system Plasmid pJW566 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
| 点击此处可从《微生物学报》下载免费的PDF全文 |
