兔出血症病毒2型理化特性研究

【目的】本研究旨在研究该病毒的理化特性,评价不同处理条件对RHDV2的杀灭效果。【方法】本研究拟对临床疑似RHDV2感染致死的家兔进行RT-PCR鉴定病原,并利用不同pH值、不同温度、常用兽用消毒剂、不同浓度甲醛处理RHDV2,通过PMA-RT-qPCR对病毒理化特性进行研究。【结果】经RT-PCR检测与测序分析,确诊为RHDV2感染,该毒株VP60基因序列与GenBank中RHDV2(登录号:MN276176.1)一致性为98.35%。PMA-RT-qPCR结果表明,RHDV2对酸碱、高温、兽用消毒剂、甲醛均表现不同程度的敏感性。酸碱处理病毒,随pH值降低或升高,病毒杀灭率均有提高。65℃处理病毒30 min后,病毒杀灭率可达77.61%,83℃处理5 min后,病毒杀灭率可达95.10%。RHDV2对3种所测兽用消毒剂均敏感,其中聚维酮碘对RHDV2杀灭作用最好,作用30 min后,病毒杀灭率达88.78%。0.3%甲醛的杀灭率优于0.2%甲醛,作用30 min后,其杀灭率可达82.22%。【结论】本研究探究了RHDV2的理化特性,该病毒对酸碱、高温、兽用消毒剂、甲醛均有不同程度的敏感性。本研究为RHDV2的诊断、临床消毒剂的选择提供了参考依据,为该病毒致病机理研究与疫苗研发奠定基础。

Physicochemical properties of rabbit hemorrhagic disease virus type 2

Objective]The purpose of this study was to study the physicochemical properties of the virus RHDV2 and evaluate effect of different treatment to kill the virus.Methods]The virus was identified by RT-PCR in rabbits with clinical suspected death from RHDV2 infection.The physicochemical properties of RHDV2 were studied by PMA-RT-qPCR at different pH,different temperatures,common veterinary disinfectants and different concentrations of formaldehyde.Results]RHDV2 infection was confirmed by RT-PCR detection and sequencing analysis.The VP60 gene sequence of this strain was 98.35%consistent with RHDV2 in GenBank(MN276176.1).PMA-RT-qPCR results show that RHDV2 was sensitive to pH,high temperature,animal disinfectant and formaldehyde.The killing rate of virus increased with pH decreasing or increasing.The virus killing rate reached 77.61%after being treated at 65℃for 30 min,and 95.10%after being treated at 83℃for 5 min.RHDV2 is sensitive to all three animal disinfectants,among which povidone iodine had the best killing effect on RHDV2,the virus killing rate reached 88.78%after 30 min.The killing rate of 0.3%formaldehyde was better than 0.2%,and after 30 min of application,the killing rate could reach 82.22%.Conclusion]RHDV2 is sensitive to acid and base,high temperature,animal disinfectant and formaldehyde to varying degrees.