| 黑曲霉蛋白酶缺陷突变体的分离和鉴定 |
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| 引用本文： | 洪斌,张洋,李元.黑曲霉蛋白酶缺陷突变体的分离和鉴定[J].微生物学报,2000,40(4):400-405. |
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| 作者姓名： | 洪斌 张洋 李元 |
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| 作者单位： | 中国医学科学院协和医科大学医药生物技术研究所北京 100050 |
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| 基金项目： | 国家高技术计划生物技术领域青年科学基金资助项目(863)(227) |
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| 摘 要： | 对黑曲霉(Aspergillus niger)3.795进行紫外诱变和筛选，获得了胞外蛋白酶缺失的菌株。其中突变株37951、3795123和3795130的生长特性与原株基本相同，胞外蛋白酶的活性明显低于原株，葡萄糖淀粉酶的活性与原株基本相同。突变株3795123和3795130的胞外蛋白酶活性分别为原株的54%和84%，可作为外源基因高效分泌表达的宿主菌。
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| 关 键 词： | 黑曲霉， 胞外蛋白酶， 蛋白酶缺失菌株， 外源蛋白降解 |
| 文章编号： | 0001-6209(2000)04-0400-06 |
ISOLATION AND CHARACTERIZATION OF MUTANTS OF ASPERGILLUS NIGER DEFICIENT IN EXTRACELLULAR PROTEASES |
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| Hong Bin,Zhang Yang,Li Yuan.ISOLATION AND CHARACTERIZATION OF MUTANTS OF ASPERGILLUS NIGER DEFICIENT IN EXTRACELLULAR PROTEASES[J].Acta Microbiologica Sinica,2000,40(4):400-405. |
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| Authors: | Hong Bin Zhang Yang Li Yuan |
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| Institution: | Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100050. |
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| Abstract: | Several mutants of Aspergillus niger 3 795 , deficient in extracellular protease expression, have been isolated and characterized biochemically after in vivro UV mutagenesis of conidiospores and selected by halo screening on the gelatin/casein plate and skim milk plate. Extracellular proteolytic activities of 3 795 1 23 and 3 795 1 30 are 5.4% and 8.4% of the parental strain and these strains may be used as the host for efficient expression of foreign proteins. |
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| Keywords: | Aspergillus niger Extracellular proteases Protease deficient mutants Heterologous protein degradation |
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