| 鼠疫菌V抗原基因在大肠杆菌中的克隆及表达 |
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| 引用本文： | 傅林锋,王秉翔,李启明,雷清,蒋琳,沈心亮.鼠疫菌V抗原基因在大肠杆菌中的克隆及表达[J].微生物学报,2003,43(4):430-433. |
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| 作者姓名： | 傅林锋 王秉翔 李启明 雷清 蒋琳 沈心亮 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 从鼠疫杆菌野毒株总DNA中利用PCR方法扩增出V抗原编码基因，将此基因克隆到大肠杆菌的表达质粒pET42b(+)中，经IPTG诱导后，在大肠杆菌BL21(DE3)细胞中成功地表达出鼠疫菌V抗原蛋白。目的蛋白表达量占菌体总蛋白的32.8%。
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| 关 键 词： | 鼠疫杆菌， V抗原， 基因克隆与表达 |
| 文章编号： | 0001-6209(2003)04-0430-04 |
Cloning and Expression of Yersinia pestis V Antigen in Escherichia coli BL21(DE3) |
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| Fu Linfeng Wang Bingxiang,Li Qiming Lei Qing Jiang Lin Shen Xinliang.Cloning and Expression of Yersinia pestis V Antigen in Escherichia coli BL21(DE3)[J].Acta Microbiologica Sinica,2003,43(4):430-433. |
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| Authors: | Fu Linfeng Wang Bingxiang Li Qiming Lei Qing Jiang Lin Shen Xinliang |
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| Institution: | Lanzhou Institute of Biological Products, Lanzhou 730046, China. linfengfu@yahoo.com.cn |
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| Abstract: | The structure gene of V antigen was amplified from the wild strain of Yersinia pestis by means of PCR, and was cloned into vector pET-42b(+). Then, V antigen was successfully expressed in E. coli BL21(DE3) with the induction of IPTG. The expressing amount of the aim protein is as high as 32.8 percent of the total bacterial protein. |
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| Keywords: | Yersinia pestis V antigen Gene cloning and expression |
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