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副溶血弧菌海产品分离株tdh基因及其临近区域结构分析
引用本文:王淑娜,Khamphouth VONGXAY,沈飚,周向阳,金培婕,陈健舜,方维焕.副溶血弧菌海产品分离株tdh基因及其临近区域结构分析[J].微生物学报,2009,49(12):1576-1583.
作者姓名:王淑娜  Khamphouth VONGXAY  沈飚  周向阳  金培婕  陈健舜  方维焕
作者单位:1. 浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,杭州,310029;舟山出入境检验检疫局综合技术服务中心,舟山,316000
2. 浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,杭州,310029
3. 舟山出入境检验检疫局综合技术服务中心,舟山,316000
基金项目:国家自然科学基金(30571436)
摘    要:摘要:【目的】初步探索副溶血弧菌海产品分离株tdh基因区域的结构特征。【方法】采用长距离PCR和基因步移技术进行tdh基因侧翼序列扩增,测序验证后拼接成疑似毒力基因片段,将所获序列与NCBI数据库进行比较,初步明确tdh基因侧翼序列的结构与功能。【结果】海产品分离株ZS34与参考菌株 RIMD2210633的tdh基因区域(VPA1310-VPA1327)结构基本一致,核苷酸同源性达98.3%;而FJ14与WZ64株基因组中的tdh基因均与tdh3的同源性最高,在基因组中的位置也不同于ZS34株和参考菌株

关 键 词:关键词:副溶血弧菌,tdh  基因,类插入序列,基因水平转移
收稿时间:2009/8/18 0:00:00
修稿时间:2009/9/12 0:00:00

Analysis of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods
Shuna Wang,Khamphouth VONGXAY,Biao Shen,Xiangyang Zhou,Peijie Jin,Jianshun Chen and Weihuan Fang.Analysis of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods[J].Acta Microbiologica Sinica,2009,49(12):1576-1583.
Authors:Shuna Wang  Khamphouth VONGXAY  Biao Shen  Xiangyang Zhou  Peijie Jin  Jianshun Chen and Weihuan Fang
Institution:Institute of Preventive Veterinary Medicine, Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 310029, China;Institute of Preventive Veterinary Medicine, Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 310029, China;Zhoushan Entry-Exit Inspection And Quarantine Bureau, Zhoushan 316000, China;Zhoushan Entry-Exit Inspection And Quarantine Bureau, Zhoushan 316000, China;Institute of Preventive Veterinary Medicine, Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 310029, China;Institute of Preventive Veterinary Medicine, Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 310029, China;Institute of Preventive Veterinary Medicine, Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, 310029, China
Abstract:Abstract: Objective] To analyze the structural characteristics of tdh gene and its adjacent loci of Vibrio parahaemolyticus isolates from seafoods. Methods] Long distance PCR and genome walking were used to amplify the DNA sequences flanking the tdh gene, and the sequences were analyzed by blastn against the NCBI database. Results] The genetic structure of tdh-adjacent loci (VPA1312-VPA1327) from isolate ZS34 was similar to that of the reference strain RIMD2210633, with the nucleotide identity of 98.3%. The tdh gene of isolates FJ14 and WZ64 was located in the loci different from the reference strain and ZS34, and showed high nucleotide similarity to tdh3 gene. In the genome of isolate FJ14, tdh was 15kb away from the trh-ure cluster, with IS-like elements and transposase genes inserted therein. Isolate WZ64 lacked trh gene, but also harbored IS-like elements upstream of tdh. Conclusion] The tdh-adjacent loci of V. parahaemolyticus seafood isolates exhibit high diversity, an additional evidence of lateral gene transfer in this particular species.
Keywords:Key words: Vibrio parahaemolyticus  tdh gene  IS-like elements  Lateral gene transfer
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