用标记分析法对我国海南地区登革2型病毒株的抗原表位分析

用标记分析(signature analvsis)法了解我国海南地区3年(1985—1987)来流行的登革2型(DEN-2)病毒株的抗原性变化。用3种单克隆抗体(McAb)，包括黄病毒属特异、亚属特异和登革2型型特异，分析了8个DEN-2病毒流行株，并与标准株新几内亚B林进行比较．通过统计分析和微机处理，发现8株中有5株与株准株类似，有3株显示出明显差异。株记分析法为病毒抗原性分析提供了一个高度敏感的方法，并可用于监测一个地区病毒株群的变化及新株的引人。

Antigen signature analysis of dengue-2 viruses strains in Hainan China

In this paper, we try to define the extent of antigenic variation between dengue-2 viruses from Hainan province, which had been isolated over a period of 3 years (1985-1987). The dengue-2 viruses were compared with the prototype New Guinea B strain and were subjected to antigen signature analysis. Eight strains of dengue-2 virus were analyzed by three monoclonal antibodies: flavivirus group, subcomplex dengue-2 type-specific reactive epitopes, over a range of antigen concentration. Five out of eight dengue-2 virus strains showed them to be antigenically homogeneous, and the remaining three strains showed them to be heterogeneous. Signature analysis provides a rapid and simple means to different strains derived from different sources, thus permit monitoring changes or introductions of hew dengue virus populations in certain geographic region.