分枝杆菌全合成琼脂培养基的研究

本文报道了一种适合常见致病分枝杆菌生长的全合成固体琼脂培养基体系。它为实验室研究分枝杆菌的营养生理、生化特性、遗传变异、药理和耐药机制提供了一种新工具，也为研制更加满意的分枝杆菌培养基创造了条件。试验过的大多数非典型分枝杆菌，在全合成琼脂培养基上比在罗氏培养基上生长快，生长量少于或等于罗氏培养基。标准牛型株的细胞群体中，仅有少量细胞能在全合成琼脂培养基上生长。鸟型．胞内和偶发等分枝杆菌标准株在全合成琼脂培养基上产生两种不同的菌落。

A study on completely defined agar media for mycobacteria

A serial of completely defined agar media suitable for growth of the common mycobacteria which are pathogenic was reported in this paper. A tool was supplied for the study of mycobacteria on nutrition and physiology, biochemical feature, genetics and variation and on the mechanism of resistance and susceptibility to antimicrobial drug, and with it would be in favour of developing more satisfactory media for mycobacteria. The majority of strains of the mycobacteria tested grew more rapidly on the completely defined agar media than on Lowenstein-Jensen medium and the final yield of the microbes on the agar media was less than or equal to those on Lowenstein-Jensen medium. There were only small amounts of growing cells on the completely defined agar media among the strain of mycobacterium bovis. Tcolonies with the above mentioned twodifferent patterns were found to grow on the completely defined media from mycobacterium avium. M. intracclluare and M. fortuitum.