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药用植物内生放线菌的生物活性及菌株D62的代谢产物分析
引用本文:刘宁,张辉,郑文,黄英,王海彬;.药用植物内生放线菌的生物活性及菌株D62的代谢产物分析[J].微生物学报,2007,47(5):823-827.
作者姓名:刘宁  张辉  郑文  黄英  王海彬;
作者单位:1. 中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100101;中国科学院研究生院,北京,100049
2. 浙江海正药业技术中心,台州,318000
3. 中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100101
基金项目:浙江海正药业技术开发项目
摘    要:利用琼脂移块法及WST-8法分别对分离自西双版纳药用植物的165株内生放线菌进行了抗菌、抗肿瘤活性测定。结果显示,超过42%的菌株对病原菌表现出拮抗活性,且对病原真菌的总体拮抗活性明显强于土壤放线菌;78%的菌株表现出抗肿瘤活性,且大部分菌株(54.5%)具有强抗肿瘤活性。选取其中对真菌及肿瘤细胞均有高抑制活性的菌株D62,并对其次生代谢产物进行了进一步的研究,共分离得6个化合物,分别是Antimycin A4a(1),Antimycin A7a(2)、Antimycin A2a(3)、Antimycin A1a(4)、10-hydroxy-10-methyl-dodec-2-en-1,4-olide(5)及6-(2-(4-aminophenyl)-2-oxoethyl)-3,5-dimethyl-tetrahydropyran-2-one(6),其中化合物6为新化合物。以上结果表明药用植物内生放线菌作为一类新的微生物资源具有很好的开发潜力。

关 键 词:内生放线菌  抗菌活性  抗肿瘤活性  次生代谢产物  新化合物
文章编号:0001-6209(2007)05-0823-05
收稿时间:7/2/2007 12:00:00 AM
修稿时间:2007-07-02

Bioactivity of endophytic actinomycetes from medicinal plants and secondary metabolites from strain D62
LIU Ning,ZHANG Hui,ZHENG Wen,HUANG Ying and WANG Hai-bin.Bioactivity of endophytic actinomycetes from medicinal plants and secondary metabolites from strain D62[J].Acta Microbiologica Sinica,2007,47(5):823-827.
Authors:LIU Ning  ZHANG Hui  ZHENG Wen  HUANG Ying and WANG Hai-bin
Institution:1.State Key Laboratory of Microbial Resources; Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China;3.Graduate University of Chinese Academy of Sciences; Beijing 100049; China;Center of Technology; Hisun Pharmaceutical Co; .Ltd; Taizhou 318000; China;State Key Laboratory of Microbial Resources; Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China;State Key Laboratory of Microbial Resources; Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China;Center of Technology; Hisun Pharmaceutical Co; .Ltd; Taizhou 318000; China
Abstract:It is believed that genetic recombination of the endophytes with the hosts that occurred in evolutionary time could result in some endophytes producing certain phytochemical originally characteristic of the host. Based on this widely accepted hypothesis, there have been increasing research efforts focused on screening for novel natural products from endophytes. In this study, antimicrobial and antitumor activities of 165 actinomycetes isolated from medicinal plants collected from Xishuangbanna were tested by agar diffusion method and WST-8 assay respectively. The results showed that over 42% of the isolates exhibited antagonism against pathogenic strains, and 54.5% displayed excellent inhibition against mouse melanoma cell line B16 or/and human alveolar epithelial cell line A549. These results are superior to those of soil actinomycetes, indicating tremendous potential of endophytic of actinomycetes for exploration. Six compounds that had both antimicrobial and antitumor activities were separated and purified from isolate Streptomyces sp. D62 by resin adsorption, silica-gel column and sephadex chromatography, etc. On the basis of spectral analyses, they were identified as antimycin A_ 4a (1), antimycin A_ 7a (2), antimycin A_ 2a (3), antimycin A_ 1a (4), 10-hydroxy-10-methyl-dodec-2-en-1,4-olide(5) and 6-(2-(4-aminophenyl)-2-oxoethyl)-3,5-dimethyl-tetrahydropyran-2-one(6), with the last one defined as a novel compound. Based on all these results, it is convinced that endophytic actinomycetes are a promising resource for bioactive natural product discovery.
Keywords:endophytic actinomycetes  antimicrobial activity  antitumor activity  secondary metabolites  novel compound
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