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焦化废水处理系统中不同培养基分离的细菌种群多样性
引用本文:陈敏,赵立平.焦化废水处理系统中不同培养基分离的细菌种群多样性[J].微生物学报,2003,43(3):366-371.
作者姓名:陈敏  赵立平
作者单位:1. 杭州师范学院生命科学学院,杭州,310012;上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海,200240
2. 上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海,200240
基金项目:国家高科技研究发展计划项目 (SZ 0 3 0 1 0 4 ),“863计划”项目 (2 0 0 1AA2 14 131)~~
摘    要:以焦化废水处理系统的微生物群落为对象,对3种不同培养基(YPG、LB、WW)分离细菌的能力及分离物的种群多样性组成进行了比较研究。同一悬浮污泥样品在YPG、LB和WW培养基上的活菌计数结果分别为1.6×10.6 CFU/mL、7.0×10.5 CFU/mL和98×10.5CFU/mL。从每种培养基10-4稀释度平板上共分离137株分离物。将所有分离物扩增近全长的16S rDNA并用限制性内切酶HinfI对PCR产物进行ARDRA(Amplified rDNA restriction analysis)多态性分析,共得到14种不同的操作分类单元(Operational Taxonomic Unit, OUT)。其中YPG培养基上的分离物显示了8种不同的OTUs,而WW培养基和LB培养基分离物只分别显示了6种和4种OTUs。YPGOTU1和WWOTU6所包含的菌株分别占到总分离物的30%和22.3%,为优势分离物。ERICPCR基因组指纹图分析表明,前者的34株分离物共有20种不同的指纹图类型,而后者的25株分离物只有3种。因此,就分离焦化废水处理系统中的细菌及对分离物进行种群多样性的研究而言,YPG培养基比其他两种培养基更合适。

关 键 词:培养基,  分离物,  种群多样性,  ARDRA,  ERIC-PCR
文章编号:1006-6179(2003)03-0366-06

Biodiversity of Bacterial Isolates on Three Different Media from Coking Wastewater Treatment System
Chen Min , Zhao Liping.Biodiversity of Bacterial Isolates on Three Different Media from Coking Wastewater Treatment System[J].Acta Microbiologica Sinica,2003,43(3):366-371.
Authors:Chen Min  Zhao Liping
Institution:School of Life Sciences, Hangzhou Nomal College, Hangzhou 310012, China.
Abstract:Bacteria from coking wastewater treatment sludge samples were directly plated on three different isolation media (YPG, LB and WW) and the biodiversity of isolates was examined with DNA fingerprinting. Plate counts with YPG, LB and WW media were 1.6 x 10(6) CFU/mL, 7.0 x 10(5) CFU/mL and 9.8 x 10(5) CFU/mL respectively. 137 single colony isolates were obtained from these three media. ARDRA with enzymes Hinfl revealed 14 operational taxonomic units (OUT) which were dominated by YPG-OTU1 group accounting for 34 isolates and WW-OTU6 group for 25 isolates. The biodiversity of isolates from these two dominant OUT groups was further investigated by a genomic fingerprinting technique, ERIC-PCR. The results indicated that there were 20 different ERIC-PCR types present among the YPG-OTU1 group and only 3 among the WW-OTU6 group. The data showed that the populations of bacteria isolated on three different media were significantly different from each other and a higher degree of genetic diversity was observed among strains isolated from YPG media. So YPG may be recommended to be used for a non-selective medium for isolating more diversified bacteria from wastewater bio-treatment systems.
Keywords:Medium  Isolate  Population diversity  ARDRA  ERIC-PCR
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