| 胶原蛋白/BMP复合材料的制备和成骨性能研究 |
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| 引用本文： | 谈伟强,徐靖宏,刘友山,林 军,王慧明.胶原蛋白/BMP复合材料的制备和成骨性能研究[J].微生物学报,2008,24(2):272-277. |
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| 作者姓名： | 谈伟强 徐靖宏 刘友山 林 军 王慧明 |
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| 作者单位： | 浙江大学医学院附属第一医院整形外科, 杭州 310003;浙江大学医学院附属第一医院整形外科, 杭州 310003;浙江大学医学院附属第一医院整形外科, 杭州 310003;浙江大学医学院附属第一医院口腔科, 杭州 310003;浙江大学医学院附属第一医院口腔科, 杭州 310003 |
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| 基金项目： | 国家教育部回国人员基金资助(2000年); 浙江省医药青年优秀人材基金资助(No. 2000QN001)。 |
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| 摘 要： | 以胶原膜(含87.5 mg I型胶原蛋白)为载体, 复合3.5 mg rhBMP-2(人基因重组骨形成蛋白-2), 制备胶原蛋白/BMP复合材料。复合材料首先在兔背阔肌中埋置, 预构新生骨组织, 并采用ALP染色、Von Kossa染色和HE染色等观察复合材料的成骨过程和组织形态。然后将形成的新骨组织游离移植修复自体下颌骨体部洞穿性缺损; 并设以胶原为载体的rhBMP-2复合骨修复材料直接修复为对照组, 骨缺损不修复组为空白组。采用X线、抗压强度、硬组织切片、四环素荧光染色、骨形态计量检查, 观察复合材料修复骨缺损的质量和效果。结果表明, 胶原蛋白/BMP复合材料在兔背阔肌中4～6周成骨, 胶原材料于3～5周降解; 成骨过程为是以软骨成骨为主的方式, 新骨形态为编织骨, 可见明显的微血管分布; 游离移植修复自体下颌骨缺损, 6周缺损区为骨性愈合, 与对照组在抗压强度(P = 0.041)、新骨量(P = 0.034)均有显著性差异。胶原蛋白/BMP复合材料在骨骼肌中形成的新生骨组织可作为供骨修复一定范围的骨缺损。
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| 关 键 词： | 胶原蛋白 骨形态发生蛋白-2 异位成骨 骨缺损 |
Preparation and Osteoinduction Capacity of Collagen/Bone Morphogenetic Protein Composites |
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| Weiqiang Tan,Jinghong Xu,Youshan Liu,Jun Lin and Huiming Wang.Preparation and Osteoinduction Capacity of Collagen/Bone Morphogenetic Protein Composites[J].Acta Microbiologica Sinica,2008,24(2):272-277. |
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| Authors: | Weiqiang Tan Jinghong Xu Youshan Liu Jun Lin and Huiming Wang |
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| Institution: | Department of Plastic Surgery, The First Affiliated Hospital, College of Mdicine, Zhejiang University, Hangzhou 310003, China;Department of Plastic Surgery, The First Affiliated Hospital, College of Mdicine, Zhejiang University, Hangzhou 310003, China;Department of Plastic Surgery, The First Affiliated Hospital, College of Mdicine, Zhejiang University, Hangzhou 310003, China;Department of Stomatology, The First Affiliated Hospital, College of Mdicine, Zhejiang University, Hangzhou 310003, China;Department of Stomatology, The First Affiliated Hospital, College of Mdicine, Zhejiang University, Hangzhou 310003, China |
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| Abstract: | Collagen/bone morphogenetic protein composites were prepared with collagen type I sponge and bone morphogenetic protein-2 (BMP-2). The composites were implanted into Latissimus dorsi muscles pouches of rabbits. Samples were studied with ALP staining, Von Kossa staining, HE staining, toluidine blue staining and CD31 histochemical labeling of microvessel. Bony samples were then used to repair mandibular defect. The effects were evaluated by X-ray, compressive strength, economycin fluorescence labeling, HE staining, toluidine blue staining and bone quantity analysis. Bone formation induced by collagen/BMP composites was found as woven bone between 4 and 6 weeks; cartilaginous osteogenesis was the main type of bone formation; microvessels could be seen in the bony tissues; and the bone defects were healed completely 6 weeks after operation. Bone formation induced by collagen/BMP composites in the muscles can be used as a donor to repair the bone defect. |
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| Keywords: | collagen bone morphogenetic protein-2 BMP-2 bone defect ectopic bone formation |
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