蔬菜-秸秆废物堆肥化中细菌群落变化研究

以蔬菜-秸秆废物为堆肥原料,在堆肥化过程中应用Biolog方法研究不同阶段细菌群落的动态变化。聚类分析与主成分分析表明,细菌群落结构在一次发酵期间发生着剧烈变化,二次发酵期间趋于稳定。能转化Biolog板上第一、二类碳源的细菌是蔬菜-秸秆废物堆肥化进程中的主要细菌种群,且与木质纤维素的转化有关;第四、六类碳源可表征堆肥中耐受高温的细菌,其中第四类碳源转化细菌与木质纤维素的降解有关,而第六类碳源转化细菌属于易降解有机物转化细菌。

Analysis of bacterial communities in vegetable and straw wastes composting by Biolog method

Bacterial communities were analyzed using Biolog method in agricultural waste composting. The results of cluster analysis and principle component analysis indicated that bacterial communities varied greatly during the first stage of composting, while began to stabilize during the second stage. Bacteria that could utilize the first and second kinds of carbon sources on Biolog plate were found to be the dominant ones during composting, which were also believed to be related with lignocellulose degradation. Thermophilic bacteria were believed to be able to metabolize the fourth and sixth kinds of carbon sources on Biolog plate. Thereinto, the fourth kinds of carbon sources were also connected with lignocellulose transformation. The bacteria utilizing the sixth kinds of carbon sources were just able to metabolize some simpler organic matters.