Akkermansia与高原牛肺水肿病相关性研究

【目的】本文通过对高原牛胃肠道菌群结构组成的分析，从微生物学角度探讨Akkermansia与高原牛肺水肿病的关系。【方法】本研究以沈阳地区健康娟姗牛为对照，以引进入拉萨半年的健康娟姗牛、拉萨本地健康黄牛以及引进入拉萨半年患肺水肿病的娟姗牛的粪便作为分析样本，采用Illumina MiSeq高通量测序技术测定样本中微生物16S rRNA基因V3–V4区序列，通过比较4种粪便样本菌群组成及丰度的差异，探讨Akkermansia与高原牛肺水肿病的相关性。【结果】Verrucomicrobia中Akkermansia在拉萨本地健康黄牛的胃肠道中的含量显著高于引进入拉萨半年的健康娟姗牛，在引进入拉萨半年患肺水肿病的娟姗牛胃肠道中的含量显著高于引进入拉萨半年的健康娟姗牛。在属水平上，沈阳地区健康娟姗牛胃肠道菌群中Akkermansia丰度占比为0.07%；引进入拉萨半年的健康娟姗牛胃肠道菌群中Akkermansia丰度占比为0.09%；拉萨本地黄牛胃肠道菌群中Akkermansia丰度占比为6.62%，是优势菌属；引进入拉萨半年的患肺水肿病的娟姗牛胃肠道菌群中Akkermansia丰度占比为11.85%，且是第一优势菌属。【结论】首次从微生物学角度探讨Akkermansia与高原牛肺水肿病的关系，为将Akkermansia丰度作为诊断肺水肿病的监测指标提供参考，但具体丰度值还有待进一步研究。

Correlation between Akkermania and pulmonary edema of plateau cattle

Objective] To investigate the relationship between Akkermansia and pulmonary edema of plateau cattle through the analysis of the gastrointestinal microbiota structure in plateau cattle. Methods] The fecal samples were collected from healthy Jersey cattle in Shenyang (control), healthy Jersey cattle which had been introduced into Lhasa for half a year, local healthy yellow cattle in Lhasa, and Jersey cattle which had been introduced into Lhasa for six months and suffered from pulmonary edema. Illumina MiSeq was used for sequencing the V3–V4 region of the 16S rRNA gene in the samples. Microbiota structure and abundance were compared among the four fecal samples, thereby elucidating the correlation between Akkermansia and the pulmonary edema. Results] The content of Akkermansia in the gastrointestinal tract of healthy local Lhasa yellow cattle was significantly higher than that of healthy Jersey cattle which had been introduced into Lhasa for half a year, and the content in Jersey cattle with pulmonary edema was significantly higher than that in healthy Jersey cattle that had been introduced into Lhasa for six months. Specifically, the abundance of Akkermansia in the gastrointestinal microbiota of healthy Jersey cattle from Shenyang, healthy Jersey cattle which had been introduced into Lhasa for half a year, local yellow cattle in Lhasa, and Jersey cattle which had been introduced into Lhasa for half a year with pulmonary edema was 0.07%, 0.09%, 6.62% (dominant genus), and 11.85% (the first dominant genus), respectively. Conclusion] This paper investigated the relationship between Akkermansia and pulmonary edema of plateau cattle for the first time by Illumina MiSeq, which may provide a reference for using the abundance of Akkermansia as an indicator for the diagnosis of pulmonary edema, although the specific abundance value needs to be further determined.