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| 硇洲岛海胆可培养细菌的多样性 | |
| 引用本文： | 黄苛,张丽,刘祝祥,陈奇辉,彭清忠,李文均,崔晓龙,陈义光.硇洲岛海胆可培养细菌的多样性[J].微生物学报,2009,49(11):1424-1429. |
| 作者姓名： | 黄苛 张丽 刘祝祥 陈奇辉 彭清忠 李文均 崔晓龙 陈义光 |
| 作者单位： | 1. 吉首大学,植物资源保护与利用湖南省高校重点实验室,生物资源与环境科学学院,吉首,416000 2. 云南大学,云南省微生物研究所,教育部微生物多样性可持续利用重点实验室,昆明,650091 3. 吉首大学,植物资源保护与利用湖南省高校重点实验室,生物资源与环境科学学院,吉首,416000;云南大学,云南省微生物研究所,教育部微生物多样性可持续利用重点实验室,昆明,650091 |
| 基金项目： | 国家自然科学基金（30860013）；国际合作重大项目（2007DFB31620）；湖南省重点学科建设项目（JSS2009Z01）; 吉首大学项目（jsdxkyzz200811, 08JDY017） |
| 摘 要： | 摘要：【目的】研究南海硇洲岛马粪海胆（Hemicentrotus pulcherrimus）可培养细菌多样性。【方法】采用纯培养法和基于16S rRNA基因序列的系统发育分析对样品中细菌（含放线菌）多样性进行研究。【结果】用补充0～2.0 mol/L NaCl的MA、ISP 2、NA、SWA和HAA培养基从海胆样品中分离到106株细菌菌株，根据形态观察和部分生理生化实验结果去冗余，选取34个代表性菌株进行基于16S rRNA基因序列的系统发育多样性分析。结果表明，这些分离菌株代表21个物种，属于3个大的系 |
| 关 键 词： | 关键词：马粪海胆 可培养细菌 16S rRNA基因 系统发育分析 多样性 |
| 收稿时间： | 5/4/2009 12:00:00 AM |
| 修稿时间： | 8/9/2009 12:00:00 AM |
Diversity of culturable bacteria associated with the sea urchin Hemicentrotus pulcherrimus from Naozhou Island |
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| Ke Huang,Li Zhang,Zhuxiang Liu,Qihui Chen,Qingzhong Peng,Wenjun Li,Xiaolong Cui and Yiguang Chen.Diversity of culturable bacteria associated with the sea urchin Hemicentrotus pulcherrimus from Naozhou Island[J].Acta Microbiologica Sinica,2009,49(11):1424-1429. | |
| Authors: | Ke Huang Li Zhang Zhuxiang Liu Qihui Chen Qingzhong Peng Wenjun Li Xiaolong Cui and Yiguang Chen |
| Institution: | Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China;Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China;Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China;Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China;Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, College of Biological Resource and Environment Science, Jishou University, Jishou 416000, China;Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China |
| Abstract: | |
| Keywords: | Keywords: Hemicentrotus pulcherrimus cultivable bacteria 16s rRNA gene phylogenetic analysis bacterial diversity |
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