| 紫外诱变改良柑橘采后病害拮抗菌柠檬形克勒克酵母研究 |
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| 引用本文： | 向家云,邓伯勋,刘昱佳,刘慧敏,俎延喜,余 慧.紫外诱变改良柑橘采后病害拮抗菌柠檬形克勒克酵母研究[J].微生物学报,2008,35(1):82-86. |
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| 作者姓名： | 向家云 邓伯勋 刘昱佳 刘慧敏 俎延喜 余 慧 |
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| 作者单位： | 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070;华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070;华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070;华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070;华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070;华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/国家柑橘育种中心 武汉 430070 |
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| 基金项目： | 国家自然科学基金项目资助 (No.NNSFC-30371008); 农业结构调整重大技术研究专项资助(No.04-09-01B) |
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| 摘 要： | 以柠檬形克勒克酵母(Kloeckera apiculata)为出发菌株, 采用紫外线(UV)、UV+LiCl等方法对其进行诱变改良。确定了处理的最佳剂量：紫外处理15 W 30 cm照射20 s, UV+LiCl处理UV照射20 s并在平板中加入LiCl 0.3% (W/V)。选育到一株生理特性有明显改善的变异菌株UV20-13, 果实试验中, 7 d后柑橘青、绿霉病的发病率分别比出发菌株降低25.56%和10.00%。生长动态测定和传代试验表明, 该菌株生长特性优于出发菌株, 连续传代10代, 该菌株没有出现退化、回复突变等, 在遗传上是稳定的。
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| 关 键 词： | 柑橘 采后病害 柠檬形克勒克酵母 诱变育种 紫外线 |
Ultraviolet Mutation Breeding Aspect of the Biological Control of Postharvest Diseases by Kloeckera apiculata in Citrus Fruit |
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| XIANG Jia-Yun,DENG Bo-Xun,LIU Yu-Ji,LIU Hui-Min,ZU Yan-Xi and YU Hui.Ultraviolet Mutation Breeding Aspect of the Biological Control of Postharvest Diseases by Kloeckera apiculata in Citrus Fruit[J].Acta Microbiologica Sinica,2008,35(1):82-86. |
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| Authors: | XIANG Jia-Yun DENG Bo-Xun LIU Yu-Ji LIU Hui-Min ZU Yan-Xi and YU Hui |
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| Institution: | National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070;National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070;National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070;National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070;National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070;National Centre of Citrus Breeding, Key Laboratory of Horticulture Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070 |
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| Abstract: | A series of experiments were conducted to study the mutation of Kloechera apiculata by many kinds of treatments such as UV and UV+LiCl. The optimal dosage disposal was determined: 15 W 30 cm under Ultraviolet irradiation for 20 s, UV+LiCl under Ultraviolet irradiation for 20 s and added LiCl 0.3% (w/v). One strain (UV20-13) which had obvious physiological characteristic was obtained. the incidence of blue and green mold of citrus was reduced by 25.56% and 10.00% in vivo experiment after 7 days respectively. The strain UV20-13 was tested by the experiments of subculture and dynamic growth, and the results showed that the strain UV20-13 was better than K.apiculata in the growth characteristics, and it did not appear retrogression, reversion mutation ect after subculturing 10 generations. Therefore the strain UV20-13 had genetic stability. |
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| Keywords: | Citrus Postharvest diseases Kloeckera apiculata Mutation breeding Ultraviolet radiation |
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