| 杀鞘翅目苏云金芽孢杆菌新菌株及其杀虫剂的研究 |
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| 引用本文： | 高梅影,李荣森,戴顺英,李小刚,傅建红.杀鞘翅目苏云金芽孢杆菌新菌株及其杀虫剂的研究[J].微生物学报,1999,39(6):515-520. |
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| 作者姓名： | 高梅影 李荣森 戴顺英 李小刚 傅建红 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉 430071；新疆自治区农业科学院 乌鲁木齐 830000 |
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| 基金项目： | 国家“八五”及“九五”攻关计划项目 |
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| 摘 要： | 从中国土壤中分离出2株杀鞘翅目昆虫的苏云金芽孢杆菌(Bacillus thuringiensis) YM03及SHQ11-10。YM03的血清型为H8a8b,SHQ1110的H血清型未知。二菌株皆产近菱形的薄扁伴孢晶体，分别含68～70kD和65kD的晶体蛋白质。毒力生物测定证明对柳蓝叶甲(Plagiodera versicolora)及马铃薯甲虫(Leptinotarsa decemlineata)有高毒效。发酵性能良好。YM03粉剂田间防治马铃薯甲虫有高效。稀释400倍喷雾，防治效果达94.6%。
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| 关 键 词： | 苏云金芽孢杆菌，鞘翅目，伴孢晶体 |
NEW COLEOPTERANCIDAI STRAINS OF BACILIUS THURINGIENSIS AND PRODUCTION OF COLEOPTERANCIDE |
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| Gao Meiying,\ Li Rongsen,\ Dai Shuying,\ Li Xiaogang,\ Fu Jianhong.NEW COLEOPTERANCIDAI STRAINS OF BACILIUS THURINGIENSIS AND PRODUCTION OF COLEOPTERANCIDE[J].Acta Microbiologica Sinica,1999,39(6):515-520. |
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| Authors: | Gao Meiying \ Li Rongsen \ Dai Shuying \ Li Xiaogang \ Fu Jianhong |
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| Institution: | Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071. |
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| Abstract: | Two coleopterancidal strains of Bacillus thuringiensis, YM-03 and SHQ11-10, were isolated from soil in China. H-Serotype of YM-03 was H8a8b, and H-Serotype of SHQ11-10 has not been identified. Both of them produced flat bipyramid-like crystals which consisted of 68-70 kD and 65 kD protein respectively. Bioassay indicated that these two Bt strains were very active on the larva of Leptinotarsa decemlineata and Plagioder aversicolora. They grew prosperously in fermentation. Powder of YM-03 product was high effect against colorado potato beetle in the field experiment. |
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| Keywords: | Bacillus thuringiensis Coleoptera Parasporal crystal |
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