| 林可链霉菌转化系统的建立 |
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| 引用本文： | 张惠展,姚峰,叶江.林可链霉菌转化系统的建立[J].微生物学报,1999,39(1):80-83. |
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| 作者姓名： | 张惠展 姚峰 叶江 |
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| 作者单位： | 华东理工大学生化工程系微生物教研室 上海 200237 |
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| 基金项目： | 国家自然科学基金,国家教委优秀青年教师基金 |
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| 摘 要： | 利用基因工程技术改良抗生素生产菌有着广阔的应用前景［１］。值得尝试的是将抗生素生物合成基因片段随机或定向组合导入生产菌中，通过同源重组方式提高生物合成途径中限速步骤相关基因的剂量，以达到提高生产菌发酵单位的目的。此战略被作者称为“基因回转化技术”，并...
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| 关 键 词： | 林可链霉菌，原生质体再生，转化 |
ESTABLISHMENT OF TRANSFORMATION SYSTEM OF STREPTOMYCES LINCOLNENSIS |
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| Zhang Huizhan\ Yao Feng\ Ye Jiang.ESTABLISHMENT OF TRANSFORMATION SYSTEM OF STREPTOMYCES LINCOLNENSIS[J].Acta Microbiologica Sinica,1999,39(1):80-83. |
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| Authors: | Zhang Huizhan\ Yao Feng\ Ye Jiang |
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| Institution: | Department of Biochemical Engineering, East China University of Science and Technology, Shanghai 200237. |
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| Abstract: | Transformation system of S. lincolnensis was established to improve lincomycin--producing strains by Gene retransformation Technology. Under optimal conditions, 10(11) protoplasts were prepared from 25 ml SM culture medium, and the regeneration frequency of protoplasts was 16%. The transformation frequency of pIJ702 was up to 5.5 x 10(5)/microgram DNA. The results revealed that gene manipulation in S. lincolnensis was facile as that in S. lividans. |
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| Keywords: | Streptomyces lincolnensis Protoplast regeneration Transformation |
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