| 黄瓜花叶病毒山东株（CMV—SD）RNA2全和cDNA克隆的构建及全序列分析 |
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| 引用本文： | 张国华,许燕.黄瓜花叶病毒山东株（CMV—SD）RNA2全和cDNA克隆的构建及全序列分析[J].微生物学报,1999,39(1):8-14. |
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| 作者姓名： | 张国华 许燕 |
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| 摘 要： | 分别利用５’ＲＡＣＥ和３’ＲＡＣＥ确定了ＣＭＶ－ＳＤＲＮＡ２的５’和３’未端序列,在此基础上,利用ＲＴ－ＰＣＲ得到了ＲＮＡ２的５’端一半的ｃＤＮＡＤＱ ＢＴＧ Ｐｃ２５和３’端一半的ｃＤＮＡ克隆ＰＣ２３,并通过拼接构建了ＲＮＡ２全长ｃＤＮＡ克隆ＰＣ２Ｆ。
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| 关 键 词： | 黄瓜花叶病毒 山东株 RNA2 cDNA克隆 序列分析 |
Construction of full-length cDNA clone and complete nucleotide sequence of RNA2 of cucumber mosaic virus SD strain] |
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| G Zhang,Y Xu,W Cai,R Fang.Construction of full-length cDNA clone and complete nucleotide sequence of RNA2 of cucumber mosaic virus SD strain][J].Acta Microbiologica Sinica,1999,39(1):8-14. |
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| Authors: | G Zhang Y Xu W Cai R Fang |
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| Institution: | Laboratory of Plant Biotechnology, Institute of Microbiology, Academia Sinica, Beijing 100080. |
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| Abstract: | The full-length cDNA clone of CMV-SD RNA2 was constructed from two cDNA clones, which were obtained by RT-PCR with primers synthesized based on terminal sequences determined using 5' RACE and 3' RACE, respectively; and the complete sequence was determined. It consists of 3048 nt and there are two partial overlapped ORFs. ORF1, which is located in the 5' portion (79-2652 nt), codes for 2a protein of 858aa, and GDD consensus sequence reported to be "polymerase site" was found in deduced 2a protein sequence. ORF2, which is located in the 3' portion (2414-2746 nt), codes for 2b protein of 111aa. The overall nucleotide sequence homologies with RNA2 of CMV Fny and Q strain, which are representatives of subgroups I and II, respectively, are 91.7% and 75.6%; the amino acid sequence homologies are 93.8% and 67.7% for 2a protein, and 83.0% and 51.3% for 2b protein, respectively. These results demonstrated that CMV-SD belongs to subgroup I. |
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