| 从大肠杆菌C-8制备和纯化唾液酸 |
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| 引用本文： | 钱世钧,李剑,高建梅,郭良栋,叶军.从大肠杆菌C-8制备和纯化唾液酸[J].微生物学报,1999,39(2):178-180. |
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| 作者姓名： | 钱世钧 李剑 高建梅 郭良栋 叶军 |
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| 作者单位： | 中国科学院微生物研究所，北京大学生物系，北京师范大学生物系 |
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| 基金项目： | “九五”国家科技攻关项目,微生物资源前期开发国家重点实验室资助 |
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| 摘 要： | 大肠杆菌产生的多聚唾液酸是一类线性的同聚合体，这由２００个左右的唾液酸通过α２，８酮苷键连接。我们在前文［１］中已就产多聚唾液酸的菌种筛选及产酸条件作了叙述。本文将对多聚唾液酸的水解、唾液酸的纯化及鉴别进行研究，为今后应用打下基础。１材料和方法１...
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| 关 键 词： | 大肠杆菌，唾液酸，纯化 |
PREPARATION, PURIFICATION AND IDENTIFICATION OF SIALIC ACID FROM ESCHERICHIA COLI C 8 * |
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| Qian Shijun\ Li Jian\ Gao Jianmei\ Guo Liangdong\ Ye Jun.PREPARATION, PURIFICATION AND IDENTIFICATION OF SIALIC ACID FROM ESCHERICHIA COLI C 8 *[J].Acta Microbiologica Sinica,1999,39(2):178-180. |
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| Authors: | Qian Shijun\ Li Jian\ Gao Jianmei\ Guo Liangdong\ Ye Jun |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080. |
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| Abstract: | Colominic acid produced from Escherichia coli C-8 was purified by ammonia sulfate precipitation, dialysis and concentration. After hydrolysis of colominic acid at a pH of 2.5, at 70 degrees C for 4 h, sialic acid was obtained, then purified by chromatography on Dowex1-x8. Analysis of thin-layer chromatography and absorption spectrum of sialic acid in the orcinol/Fe3+/HCl and the periodic acid/thiobarbituric acid confirmed that its purity was identical with that of standard sample. |
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| Keywords: | Escherichia coli Sialic acid Purification |
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