| 固定化青霉素V酰化酶的制备及性质 |
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| 引用本文： | 董志扬,崔福绵.固定化青霉素V酰化酶的制备及性质[J].微生物学报,1999,39(6):551-553. |
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| 作者姓名： | 董志扬 崔福绵 |
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| 作者单位： | 中国科学院微生物研究所 北京 100080 |
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| 摘 要： | 尖镰孢(Fusarium oxysporum)FP941青霉素V酰化酶经γ氧化铝吸附洗脱、硫酸铵沉淀和脱盐处理后，固定在环氧丙烯聚合物载体上，湿固定化酶表现活力为217 IU/g，固定化产率为53%。固定化酶作用最适温度为55℃，最适pH为80；在pH50～110及50℃以下稳定；37℃使用25次后，酶活力保留90%。
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| 关 键 词： | 青霉素V酰化酶，固定化，尖镰孢 |
PREPARATION OF IMMOBILIZED PENICILIN V ACYLASE FROM FUSARIUM OXYSPORUM AND ITS PROPERTIES |
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| Dong Zhiyang\ Cui Fumian.PREPARATION OF IMMOBILIZED PENICILIN V ACYLASE FROM FUSARIUM OXYSPORUM AND ITS PROPERTIES[J].Acta Microbiologica Sinica,1999,39(6):551-553. |
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| Authors: | Dong Zhiyang\ Cui Fumian |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080. |
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| Abstract: | Extracellular penicillin V acylase from Fusarium oxysporum FP941 was partially purified by means of adsorption on, and elution from gamma-alumina and (HN4)2SO4 fractional precipitation. The enzyme was immobilized on acrylic polymer support linkaged with covalent bond. The activity of wet immobilized enzyme was 217 IU/g, The activity yield of Immobilized enzyme was 53%. Optimum pH and temperature for immobilized enzyme action wer 8.0 and 55 degrees C, respectively. The enzyme was stable below 50 degrees C and pH 4-11. The activity of the immobilized enzyme remained 90% after it was reused 25 times. |
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| Keywords: | Penicillin V acylase Immobilization Fusarium oxysporum |
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