氮离子注入对耐辐射异常球菌SOD活性的影响及其对Mn—SOD的诱导

研究了氮离子注入对耐辐射异常球菌（Ｄｅｉｎｏｃｏｃｃｕｓｒａｄｉｏｄｕｒａｎｓ）细胞内超氧化物歧化酶（ＳＯＤ）活性的影响及其对ＭｎＳＯＤ的诱导。结果表明，当２０ｋｅＶ氮离子的注入剂量低于８×１０１４ｉｏｎｓ／ｃｍ２时，Ｄ．ｒａｄｉｏｄｕｒａｎｓ中ＳＯＤ活性变化不大，当剂量在８×１０１４～６０×１０１４ｉｏｎｓ／ｃｍ２范围内时，ＳＯＤ的活性随着注入剂量的增大逐渐提高，但大于６０×１０１４ｉｏｎｓ／ｃｍ２时，则逐渐下降；加入对不同金属辅基的ＳＯＤ同工酶活性抑制剂Ｈ２Ｏ２和氯仿乙醇的研究表明，中高剂量下氮离子注入诱导的是Ｄ．ｒａｄｉｏｄｕｒａｎｓ中ＭｎＳＯＤ活性的提高，在正常生理条件及小于８×１０１４ｉｏｎｓ／ｃｍ２的剂量下，Ｄ．ｒａｄｉｏｄｕｒａｎｓ中ＳＯＤ总活性主要由ＦｅＳＯＤ构成

THE EFFECT OF N IMPLANTATION ON SOD ACTIVITY OF DEINOCOCCUS RADIODURANS AND INDUCTION OF Mn SOD

The induction of Mn-SOD and effect of N+ of 20 keV implantation on SOD activity of D. radiodurans were investigated. The results were as follows: (1) SOD activity of D. radiodurans had a little change when implantation dose was less than 8 x 10(14) N+/cm2, and increased gradually from 8 x 10(14) to 60 x 10(14) N+/cm2, and then decreased rapidly; (2) the test of adding H2O2 and CHCl3-CH3CH2OH which could inhibit different types of SOD showed that most SOD activity of D. radiodurans was constituted by Fe-SOD in general physiology and less then 8 x 10(14)N+/cm2 dose, and higher implantation dose induced increase of Mn-SOD activity.