| 活性氧对苏云金芽孢杆菌伴孢晶体的损伤作用 |
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| 引用本文： | 王文军,钱传范,申继忠,杨韶松.活性氧对苏云金芽孢杆菌伴孢晶体的损伤作用[J].微生物学报,1999,39(5):469-474. |
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| 作者姓名： | 王文军 钱传范 申继忠 杨韶松 |
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| 作者单位： | 中国农业大学应用化学系 北京 100094 |
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| 摘 要： | 用SDSPAGE电泳分析和生物测定方法研究了过氧化氢(H2O2)和羟自由基(·OH)对苏云金芽孢杆菌(Bacillus thuringiensis)伴孢晶体的损伤作用。结果表明，这两种活性氧对伴孢晶体均有一定程度的损伤作用，这种损伤作用与活性氧的浓度成正相关，并且·OH对伴孢晶体的损伤作用明显强于H2O2。
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| 关 键 词： | 过氧化氢，羟自由基，苏云金芽孢杆菌伴孢晶体 |
THE EFFECTS OF REACTIVE OXIDANTS ON BACILLUS THURINGIENSIS PARASPORAL CRYSTALS |
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| Wang Wenjun,Qian Chuanfan,Shen Jizhong Yang Shaosong.THE EFFECTS OF REACTIVE OXIDANTS ON BACILLUS THURINGIENSIS PARASPORAL CRYSTALS[J].Acta Microbiologica Sinica,1999,39(5):469-474. |
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| Authors: | Wang Wenjun Qian Chuanfan Shen Jizhong Yang Shaosong |
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| Institution: | Department of Applied Chemistry, China Agricultural University, Beijing 100094. |
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| Abstract: | The effects of hydrogen peroxides(H2O2) and hydroxyl radicals(.OH) on the purified parasporal crystals from Bacillus thuringiensis var. Kurstaki HD-1 were studied by SDS-PAGE and bioassay. The results showed that to some extent H2O2 and .OH produced a direct and deleterious effect. Hydroxyl radicals from the system Fe(II)-EDTA-H2O2 indicated more serious damage than hydrogen peroxides. There was a 44.5% loss in activity against 12-h-old larvae of Heliothis armigera when parasporal crystals were treated with 0.3% H2O2 only. However, 85.2% activity lost when parasporal crystals were treated with 0.3% H2O2 in the presence of iron(II) and EDTA. |
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| Keywords: | Hydrogen peroxide Hydroxylradical Bacillus thuringiensis parasporal crystal |
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